摘要
【目的】探讨低温胁迫下外源喷施山梨醇对枇杷幼苗生长发育及抗寒性的影响。【方法】以枇杷品种‘果真好1号’幼苗为材料,研究外源山梨醇处理对低温胁迫下枇杷幼苗生理特性的影响,测定山梨醇叶面喷施处理后,枇杷幼苗在-1℃低温胁迫下第2、6、12、24、48 h的光合特性和抗寒生理生化特性。【结果】外源山梨醇处理降低了低温胁迫下枇杷幼苗的胁迫程度,山梨醇处理组叶片冻害率在第48 h较对照降低了39.64%,山梨醇含量和可溶性蛋白含量分别增加6.34%、5.22%,延缓了叶绿素和类胡萝卜素的降解及叶绿素荧光的增加,维持了叶片光合作用,同时缓解了膜损伤程度,使相对电导率降低37.80%,增加了抗氧化酶(SOD、POD、APX、CAT)活性。【结论】低温胁迫下外源山梨醇可以减少低温胁迫对枇杷叶片叶绿色素含量、光合作用、光合系统和抗氧化系统的影响,缓解枇杷幼苗的低温胁迫伤害,提高枇杷幼苗的抗寒能力。
【Objective】To evaluate the effects of exogenous sorbitol on the growth and cold tolerance in loquat(Eriobotrya japonica)seedlings under low temperature stress.【Method】This experiment utilized‘Guozhenhao No.1’loquat seedlings as plant materials to investigate the impact of exogenous sorbitol treatment on their physiological characteristics under low temperature stress.The physiological and biochemical properties related to photosynthetic and cold resistance were measured in loquat seedlings treated with water and sorbitol leaf spraying were measured at 2,6,12,24,and 48 hours under-1℃low temperature conditions.【Result】Exogenous sorbitol treatment reduced the stress degree of loquat seedlings under low temperature stress.The leaf frost damage rate in the sorbitol treatment group decreased by 39.64%at the 48th hour compared with the control group,while sorbitol content and soluble protein content increased by 6.34%and 5.22%,respectively.The treatment delayed the degradation of chlorophyll and carotenoids,increase of chlorophyll fluorescence,maintained the photosynthesis of leaves.It also alleviated the degree of membrane damage,reduced the relative electrical conductivity by 37.80%,and enhanced the activities of antioxidant enzymes(SOD,POD,APX,CAT)were increased.【Conclusion】Under low temperature stress,exogenous sorbitol can reduce the effects on the chlorophyll content,photosynthesis,photosynthetic system and antioxidant system in loquat leaves.This treatment alleviates the low temperature stress damage of loquat seedlings,and improves their cold resistance.
作者
任艺华
何宛珣
杜莹
易鹏翰
牛洁
汪红霞
王雨桐
邓群仙
张慧芬
REN Yihua;HE Wanxun;DU Ying;YI Penghan;NIU Jie;WANG Hongxia;WANG Yutong;DENG Qunxian;ZHANG Huifen(College of Horticulture,Sichuan Agricultural University,Chengdu 611130,China)
出处
《四川农业大学学报》
北大核心
2025年第6期1416-1423,共8页
Journal of Sichuan Agricultural University
基金
四川省科技支撑计划“十四五”育种攻关(2021YFYZ0023-07)
国家现代化农业产业技术体系四川水果创新团队(SCCXTD-2024-04)。
关键词
山梨醇
低温胁迫
枇杷幼苗
生理特性
sorbitol
low temperature stress
loquat seedlings
physiological characters
