摘要
目的通过系统评价和实验验证,探讨微小RNA(miR)-15b-3p与卵巢储备功能减退(DOR)发生发展的关系。方法计算机检索PubMed、Cochrane Library、Embase、Web of Science、万方、中国知网、中国生物医学文献数据库,获取各数据库建库至2024年11月27日miRNA与DOR相关文献,根据纳入与排除标准筛选文献后,对纳入文献进行数据提取及质量评价,通过汇总、筛选、分析获得与DOR发生相关的miRNA。随后选取2024年3月至2024年12月在佛山复星禅诚医院中医妇科就诊的DOR患者和正常卵巢储备功能(NOR)患者为研究对象,检测患者血浆miR-15b-3p表达水平。结果共获得miRNA与DOR相关文献1626篇,最终纳入文献8篇,共涉及研究对象125人,其中实验组共62人,对照组共63人。8篇纳入文献共涉及10个独立研究、5种样本类型,结果共鉴定出94个miRNA至少在两项实验中差异表达。纳入文献的纽卡斯尔-渥太华量表(NOS)平均得分7.75分。临床检测结果显示,14位DOR患者和35位NOR患者血浆样本比较,DOR组患者血浆miR-15b-3p表达水平明显降低(P<0.001)。结论miR-221-3p、miR-483-3p、miR-126-5p、miR-15b-3p、miR-140-3p等miRNA在不同地区、样本来源的DOR患者中均差异表达,其中miR-15b-3p与DOR的发生发展密切相关,可能成为DOR早期检测和靶向治疗的潜在靶点。
Objective To explore the relationship between microRNA(miR)-15b-3p and the oc-currence and development of diminished ovarian reserve(DOR)through systematic review and experimental validation.Methods Computerized searches were conducted on PubMed,Cochrane Library,Embase,Web of Science,Wanfang,CNKI,and Chinese Biomedical Literature Database(CBM)to retrieve literature on miR-NAs and DOR from database inception until November 27,2024.Following screening based on inclusion and exclusion criteria,data extraction and quality assessment were performed on the included studies.Relevant miR-NAs associated with DOR were identified through summarization,screening,and analysis.In addition,DOR patients and patients with normal ovarian reserve(NOR)who attended the Department of Traditional Chinese Medicine Gynecology at Foshan Fosun Chancheng Hospital between March 2024 and December 2024 were selected as the study subjects,and plasma miR-15b-3p expression levels were measured in both groups.Results A total of 1626 articles related to miRNAs and DOR were retrieved,and 8 articles were finally in-cluded,involving 125 subjects(62 in the experimental group and 63 in the control group).These 8 articles in-cluded 10 independent studies and 5 sample types.94 miRNAs were differentially expressed in at least 2 experi-ments.The average Newcastle-Ottawa Scale(NOS)quality score of the included studies was 7.75.Clinical vali-dation revealed that plasma miR-15b-3p levels were significantly lower in DOR patients(n=14)compared with NOR participants(n=35)(P<0.001).Conclusion MiRNAs such as miR-221-3p,miR-483-3p,miR-126-5p,miR-15b-3p,and miR-140-3p are differentially expressed in DOR patients across different regions and sample sources.Among them,miR-15b-3p is closely related to the occurrence and development of DOR and may serve as a potential biomarker and therapeutic target for early diagnosis and treatment.
作者
王颖巨
皮芳草
邬素珍
WANG Yingju;PI Fangcao;WU Suzhen(Foshan Clinical Medical School of Guangzhou University of Chinese Medicine,Foshan,Guangdong,China,528000;International Institute for Translational Chinese Medicine,Guangzhou University of Chinese Medicine,Guangzhou,Guangdong,China,510006;Foshan Fosun Chancheng Hospital,Foshan,Guang-dong,China,528000)
出处
《分子诊断与治疗杂志》
2025年第12期2310-2314,共5页
Journal of Molecular Diagnostics and Therapy
基金
广东省中医药抗衰老工程技术研究中心(2022E040)。
