摘要
通过在牛成肌细胞中过表达钙粘蛋白11(Cadherin-11,CDH11)基因并利用EDU、CCK-8、免疫荧光和实时荧光定量PCR(qRT-PCR)等技术,探讨CDH11基因对牛成肌细胞增殖与分化的作用。结果表明,过表达CDH11基因显著提高牛成肌细胞的EDU阳性率及450 nm波长下的吸光度(P<0.05),显著增强周期蛋白依懒性激酶2(Cyclin-Dependent Kinases,CDK2)和增殖细胞核抗原(Proliferating Cell Nuclear Antigen,PCNA)的表达水平(P<0.05)。在成肌细胞分化阶段,过表达CDH11基因显著减少了肌管的面积,显著抑制成肌分化相关基因成肌分化因子(Myogenic Diffeerentiation,MYOD)、肌细胞生成素(Myogenin,MYOG)和生肌调节因子5(Myogenic Regulatory Factors5,MYF5)的表达(P<0.05)。研究表明过表达CDH11基因能够促进牛成肌细胞的增殖,但对其分化具有抑制作用。
The role of cadherin-11(CDH11)gene in the proliferation and differentiation of bovine adult myoblasts was investigated by overexpressing CDH11 and using EdU,CCK-8,immunofluorescence and quantitative real-time PCR(qRT-PCR).The results showed that CDH11 overexpression significantly increased the EdU positivity and absorbance at 450 nm in bovine myogenic cells(P<0.05).The expression levels of cyclin-dependent kinase(CDK2)and Proliferating Cell Nuclear Antigen(PCNA)were significantly upregulated(P<0.05).During the myoblast differentiation stage,CDH11 overexpression significantly reduced the area of myotubes and markedly inhibited the myogenic differentiation-related genes myogenic differentiation(MYOD),myogenin(MYOG)and myogenic regulatory factors5(MYF5)expression(P<0.05).These findings indicate that CDH11 overexpression promotes the proliferation of bovine adult myoblasts but inhibits the differentiation of bovine adult myoblasts.
作者
刘占鑫
白彦斌
牛艳梅
贾雪
李亮
蔡思怡
王轶程
张军霞
刘旭辉
张晓兰
石斌刚
胡江
赵志东
应成玉
LIU Zhanxin;BAI Yanbin;NIU Yanmei;JIA Xue;LI Liang;CAI Siyi;WANG Yicheng;ZHANG Junxia;LIU Xuhui;ZHANG Xiaolan;SHI Bingang;HU Jiang;ZHAO Zhidong;YING Chengyu(College of Animal Science and Technology,Gansu Agricultural University,Key Laboratory of Herbivore Biotechnology of Gansu Province,Lanzhou 730070,China;Livestock and Veterinary Station of Mutual Aid County,Haidong Qinghai 810599,China)
出处
《西北农业学报》
北大核心
2025年第12期2175-2183,共9页
Acta Agriculturae Boreali-occidentalis Sinica
基金
甘肃省青年博士支持项目(2025QB-042)
乡村振兴专项(25CXNA058)
甘肃农业大学学科团队项目(GAU-XKTD-2022-22)。
