摘要
目的基于16S rDNA分析高血压合并冠心病患者与高血压患者肠道菌群的特点。方法收集2021年11月-2022年12月赣南医科大学第一附属医院符合纳入及排除标准的患者,将其分为高血压合并冠心病(CH组)11例,高血压(H组)22例。收集2组的基本信息、临床实验室结果、粪便标本,通过16S rDNA测序技术对33例粪便标本进行高通量测序。结果2组在总胆汁酸、硫酸氢氯吡格雷、他汀类药物上对比,差异有统计学意义(P<0.05);Alpha多样性2组对比,差异无统计学意义(P>0.05);Beta多样性显示:CH组和H组组间肠道菌群对比,差异有统计学意义(P=0.05,R^(2)=0.06);LEfSe差异分析显示:在属水平,Catabacter、Verruc_01、Dorea、Subdoligranulum富集于CH组;Spearman's相关性分析发现:Dorea、Catabacter、Subdoligranulum与他汀类药物成正相关;随机森林模型提示:他汀类药物、氯吡格雷及Catabacter作为诊断的最优参数。结论合并与不合并冠心病的高血压人群之间肠道菌群的丰度度及多样性存在差异,他汀类药物可能通过增加Subdoligranulum的丰度从而影响血脂代谢;Catabacter可能在促进高血压人群发生冠心病的过程中起着重要作用。
Objective To analyze the characteristics of intestinal flora in hypertensive patients with coronary heart disease and hypertensive patients based on 16S rDNA.Methods From November 2021 to December 2022,patients met the inclusion and exclusion criteria in the First Affiliated Hospital of Gannan Medical University were collected and divided into 11 cases of hypertension combined with coronary heart disease(CH group)and 22 cases of hypertension(H group).The basic information,clinical laboratory results and fecal specimens of the two groups were collected,and high-throughput sequencing was performed on 33 fecal specimens by 16S rDNA sequencing technology.Results There was statistically significant difference in total bile acid,clopidogrel bissulfate and statins between the two groups(P<0.05).There was no statistically significant difference in Alpha diversity between the two groups.Beta diversity showed that there was statistically significant difference in intestinal flora between group CH and group H(P=0.05,R^(2)=0.06).LEfSe analysis showed that at the genus level,Catabacte,Verruc_01,Dorea and subdoligranulum were enriched in CH group.Spearman's correlation analysis showed that Dorea,Catabacter and Subdoligranulum were positively correlated with statins.The random forest model suggested that statins,clopidogrel and Catabacter were the optimal diagnostic parameters.Conclusions There are differences in the abundance and diversity of gut microbiota between hypertensive people with and without coronary heart disease.Statins may affect lipid metabolism by increasing the abundance of rare subdoligranulum.Catabacter may play an important role in promoting coronary heart disease in hypertensive population.
作者
李昌霞
李弯
罗朝
程欢
韩啸威
程静
谢家和
LI Changxia;LI Wan;LUO Chao;CHENG Huan;HAN Xiaowei;CHENG Jing;XIE Jiahe(Department of Cardiology,Northeast Yunnan Central Hospital,Zhaotong Yunnan 657000,China;Department of Cardiology,The First Affiliated Hospital of Gannan Medical University,Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases of the Ministry of Education,Gannan Medical University,National Clinical Research Center for Geriatric Diseases,Jiangxi Branch,Ganzhou Jiangxi 341000,China;Department of General Practice,Qujiang Town Health Center of Fengcheng,Fengcheng Jiangxi 341000,China;Department of Cardiology,Changjiang River Shipping General Hospital,Wuhan Hubei 430014,China;Nanba Campus of Bazhong Central Hospital,Bazhong Sichuan 636000,China;Department of Geriatrics,731 Hospital of China Aerospace Science and Industry Corporation,Beijing 100074,China)
出处
《云南医药》
2025年第6期1-6,共6页
Medicine and Pharmacy of Yunnan
关键词
高血压
冠心病
肠道菌群
16S
rDNA测序
hypertension
coronary heart disease
intestinal flora
16S rDNA sequencing
