摘要
人工甜味剂三氯蔗糖作为糖的替代品被广泛应用,已成为环境新兴污染物。尽管近年来人们对三氯蔗糖的水生生物毒性给予一定关注,但其对土壤无脊椎动物的影响仍不明确。因此,本研究开展了不同浓度三氯蔗糖(0.2、2、20、200和2000 mg·kg^(-1))的跳虫(Folsomia candida)毒性测试,明确了个体层面(逃避行为、存活、繁殖及生长)和细胞分子层面(抗氧化酶、代谢产物变化)指标的响应特征。研究结果表明,所设浓度范围内,急性和慢性暴露后跳虫存活未受显著影响。但是,较高浓度200 mg·kg^(-1)和2000 mg·kg^(-1)处理中,逃避率、繁殖及生长抑制率显著增加。随暴露浓度的增加,跳虫体内超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性整体出现先显著升高后降低现象,而丙二醛(MDA)含量呈显著增加趋势。非靶向代谢组学测试进一步深入发现,环境相关低浓度0.2 mg·kg^(-1)处理中跳虫体内105个和144个代谢物分别出现显著上调和下调,脂质代谢(脂肪酸代谢、花生四烯酸代谢、亚油酸代谢)、氨基酸代谢(甘氨酸、丝氨酸和苏氨酸代谢)相关的通路被显著干扰。本研究初步揭示了三氯蔗糖对跳虫不同生物学水平终点产生的不利影响,可为土壤中三氯蔗糖的风险评估提供一定依据。
Sucralose,an artificial sweetener widely used as a sugar substitute,has become an emerging environmental pollutant.Although certain attention has been paid to the aquatic toxicity of sucralose in recent years,its impact on soil invertebrates remains unclear.Therefore,this study conducted toxicity tests on springtails(Folsomia candida)with different concentrations of sucralose(0.2,2,20,200,and 2000 mg·kg^(-1))to clarify the responses of indicators at the individual level(avoidance behavior,survival,reproduction,and growth),cellular and molecular l evels(changes in antioxidant enzymes and metabolites).The results showed that within the test concentrations range,the survival of springtails was not significantly affected after acute or chronic exposure.H owever,in the treatments of higher concentrations of 200 and 2000 mg·kg^(-1),the avoidance rate,and inhibition rates of reproduction and growth significantly increased.With increasing exposure concentrations,the activities of superoxide dismutase(SOD)and catalase(CAT)in springtails first increased significantly and then decreased as a whole,while malondialdehyde(MDA)content showed a significant increasing trend.Non-targeted metabolomics tests further revealed that at an e nvironmentally relevant low concentration of 0.2 mg·kg^(-1),105 and 144 metabolites in springtails were significantly up-and down-regulated,respectively.The pathways related to lipid metabolism(fatty acid metabolism,arachidonic acid metabolism,linoleic acid metabolism)and amino acid metabolism(glycine,serine,and threonine metabolism)were significantly disrupted.This study preliminarily revealed the adverse effects of sucralose on different biological level endpoints of springtails,and can provide a certain basis for the risk assessment of sucralose in soil.
作者
孙翔坤
王巍然
郭飞
徐康宁
林祥龙
SUN Xiangkun;WANG Weiran;GUO Fei;XU Kangning;LIN Xianglong(College of Environmental Science and Engineering,Beijing Forestry University,Beijing 100083,China;School of Chemical and Environmental Engineering,China University of Mining and Technology,Beijing 100083,China;State Key Laboratory of Environmental Criteria and Risk Assessment,Chinese Research Academy of Environmental Sciences,B eijing 100012,China;National Center of Standards Evaluation,State Administration for Market Regulation,Beijing 100011,China)
出处
《生态毒理学报》
北大核心
2025年第6期406-413,共8页
Asian Journal of Ecotoxicology
基金
国家重点研发计划项目(2023YFC3708700)。
关键词
三氯蔗糖
跳虫
毒性评价
土壤
代谢组学
sucralose
springtail
toxicity assessment
soil
metabolomics
