摘要
全氟和多氟烷基化合物(PFASs)对于土壤白符跳具有显著的生殖毒性。本文探究了PFASs对白符跳氧化应激和生殖相关基因表达的影响,以期从分子水平上揭示PFASs对白符跳的生殖毒性。研究结果表明,在3种PFASs(PFOA、PFBA、F-53B)诱导下,白符跳氧化应激相关的细胞色素P450单加氧酶基因(CYP6N4V1、CYP6N3V2)表达上调,其中F-53B诱导组上调幅度最大,分别为4.94倍和11.07倍;PFASs显著抑制了白符跳繁殖相关基因组织蛋白酶CTSL的表达,在PFOA、PFBA、F-53B处理下,CTSL表达量仅为对照组的64%、39%和15%。表明PFASs对土壤白符跳氧化应激及生殖相关基因的表达具有显著影响,且F-53B对白符跳相对而言具有更大的毒性。CYP6N3V2、CYP6N4V1、CTSL、VMO1和Vitrec等5个差异表达基因可作为基于PFASs暴露的土壤白符跳早期预警指标的靶标基因。
Per-and polyfluoroalkyl substances(PFASs)exhibited significant reproductive toxicity to the soil springtail Folsomia candida.This study represented an initial exploration into the oxidative stress and reproduction-related gene expression induced by PFASs and their substitutes,aiming to elucidate the molecular-level reproductive toxicity of PFASs.The findings revealed that three PFASs(PFOA,PFBA,F-53B)induced up-regulation in cytochrome P450 monooxygenase(CYP6N4V1,CYP6N3V2),genes associated with oxidative stress,with F-53B exhibiting the highest up-regulation amplitude(4.94 and 11.07 times respectively).Additionally,PFASs significantly suppressed the activity of Cathepsin L(CTSL),a gene associated with reproduction.The expression level of CTSL was observed to be only 64%,39.0%,and 15%respectively compared to that of the control group levels when exposed to PFOA,PFBA,and F-53B treatments.These results indicated that perfluorinated compounds exert substantial regulatory effects on oxidative stress and reproductive-related genes in Folsomia candida inhabiting soil environments.Five differentially expressed genes(CYP6N3V2,CYP6N4V1,CTSL,VMO1,and Vitrec)can serve as target genes for early warning indicators regarding exposure to PFASs in soil-dwelling collembolan species such as Folsomia candida.
作者
梁芳
杨扬
李柱
王孜楠
柯欣
吴峻
吴龙华
骆永明
LIANG Fang;YANG Yang;LI Zhu;WANG Zinan;KE Xin;WU Jun;WU Longhua;LUO Yongming(State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 211135,China;University of Chinese Academy of Sciences,Beijing 100049,China;Shanghai Institute for Biological and Pharmaceutical Technologies,Shanghai 200032,China;College of Resources and Environment,Southwest University,Chongqing 400716,China;Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China)
出处
《土壤》
北大核心
2025年第5期1093-1098,共6页
Soils
基金
国家自然科学基金项目(41977136,41991334)资助。
