摘要
文章以鲜乳为原料,采用100 nm与200 nm孔径陶瓷膜进行微滤,对比分析了获得的鲜乳级乳清蛋白透过液和酪蛋白截留液的蛋白质组成、蛋白脱除率和膜通量等参数。结果显示,100 nm陶瓷膜在分离乳清蛋白与酪蛋白方面效果最佳。利用10 ku陶瓷膜脱除乳糖,经浓缩和喷雾干燥,成功制备了蛋白含量达94.3%的高纯度鲜乳级乳清蛋白分离物(Milk whey protein isdation, mWPI,蛋白含量90%),进一步与干酪乳清制备的乳清蛋白浓缩物(Whey protein concentrate,WPC80,蛋白含量80%)和分离乳清蛋白(Whey protein isolation,WPI,蛋白含量90%)进行了粒径、溶解性、乳化性、起泡性及微观结构等加工特性的对比分析。结果表明,mWPI在溶解性、稳定性和起泡性上表现较好,且粉体粒径最小、表面光滑。与干酪乳清制备的WPC80和WPI相比,其起泡率和乳化性无统计学差异。为利用鲜乳通过膜分离技术工业化制备mWPI提供了技术支持。
This study used fresh milk as the raw material and compared the protein composition,protein removal rate,and membrane flux of whey protein permeate and casein retentate obtained through microfiltration with 100 nm and 200 nm ceramic membranes.The results showed that the 100 nm ceramic membrane was the most effective for separating whey protein and casein.Subsequently,lactose was removed using a 1 ku ceramic membrane,and the resulting product was concentrated and spray-dried to produce high-purity milk-derived whey protein isolate(mWPI)with a protein content of 94%.The processing characteristics,including particle size,solubility,emulsification,foaming,and microstructure,were compared with those of whey protein concentrate(WPC80,80%protein)and WPI made from cheese whey.The results indicated that mWPI had higher solubility,stability,and foaming ability,with the smallest particle size and the smoothest surface.However,its foaming capacity and emulsification properties showed no significant difference compared to WPC80 and WPI.These findings provide essential technical support for the industrial production of mWPI from fresh milk using membrane separation technology.
作者
孙正瀚
王筠钠
王琴
张鹏
吕加平
逄晓阳
张书文
李凤
刘妍妍
SUN Zhenghan;WANG Yunna;WANG Qin;ZHANG Peng;LYU Jiaping;PANG Xiaoyang;ZHANG Shuwen;LI Feng;LIU Yanyan(College of Food Science,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Karamay Lvcheng Agricultural Development Co.Ltd.,Karamay 834000,China)
出处
《中国乳品工业》
北大核心
2025年第12期21-28,共8页
China Dairy Industry
基金
国家自然科学基金项目(32302137)
新疆小组团项目(2025XZT00021)
国家奶牛产业技术体系任务(CARS-36)。
关键词
陶瓷膜分离
微滤
乳清蛋白浓缩物
加工特性
ceramic membrane separation
microfiltration
whey protein concentrate
processing characteristics
