摘要
【目的】筛选可用作定殖追踪的抗性标记,并评价优良解钾细菌在毛竹根际土壤中的定殖能力。【方法】采用氨苄青霉素、卡那霉素、壮观霉素和利福平筛选27株解钾细菌,评估抗性菌株在毛竹林灭菌与未灭菌土壤中的定殖能力。【结果】有11株菌对氨苄青霉素、卡那霉素、壮观霉素和利福平都具有抗性,9株菌全敏感。壮观霉素抗性比例最高(62.96%),卡那霉素最低(40.74%)。菌株BF69(壮观霉素)和NR33(氨苄青霉素和壮观霉素)抗性较强。在灭菌土壤中,BF69的菌落数量于30 d时达到峰值(2.37×10^(10)CFU/g干土),但在60 d时其数量显著低于NR33;在未灭菌土壤中,2个菌株存活均超过6个月。【结论】解钾细菌BF69和NR33具有优异抗性与定殖能力,上述抗生素标记可用于毛竹林土壤中解钾细菌的快速鉴定与应用开发。
[Objective]This study was conducted to screen for antibiotic resistance markers suitable for the colonization tracking and to evaluate the colonization ability of potent potassium-solubilizing bacteria(KSB)in the rhizosphere soil of moso bamboo(Phyllostachys edulis).[Method]Twenty-seven KSB strains were screened for resistance to ampicillin,kanamycin,spectinomycin and rifampicin.The colonization capacity of selected resistant strains was then assessed in both sterilized and non-sterilized moso bamboo forest soil.[Result]Among the tested strains,eleven strains exhibited resistance to at least one of the four antibiotics,while nine were susceptible to all.The resistance frequency was highest for spectinomycin(62.96%)and lowest for kanamycin(40.74%).The strains BF69(high resistance to spectinomycin)and NR33(high resistance to both ampicillin and spectinomycin)were selected for further study.In sterilized soil,the population of BF69 peaked at 30 days(2.37×10^(10) CFU/g dry soil)but was significantly lower than that of NR33 by day 60.Importantly,in non-sterilized soil,both strains successfully colonized the soil and persisted for over six months.[Conclusion]KSB strains BF69 and NR33 possess strong intrinsic antibiotic resistance and superior colonization capabilities.This intrinsic resistance serves as an effective marker for rapid identification and monitoring of KSB in moso bamboo soil,providing a strong foundation for their future application as microbial inoculants.
作者
欧阳园兰
钟曲颖
桂许维
栾丰刚
黎杰俊
OUYANG Yuanlan;ZHONG Quying;GUI Xuwei;LUAN Fenggang;LI Jiejun(Administration of Guanshan National Nature Reserve,Yichun,Jiangxi 336300,China;School of Forestry,Jiangxi Agricultural University,Nanchang 330045,China)
出处
《生物灾害科学》
2025年第4期555-561,共7页
Biological Disaster Science
基金
国家自然科学基金项目(31560203)。
关键词
解钾细菌
抗性标记
定殖
毛竹
potassium-solubilizing bacteria
resistance marker
colonization
Phyllostachys edulis
