摘要
贝莱斯芽胞杆菌JS6-1对梨火疫病、葡萄霜霉病、葡萄白粉病等作物病害均具有较好预防效果。为明确贝莱斯芽胞杆菌JS6-1的定殖特性和主要功能基因,采用抗生素标记法,研究其在杜梨、葡萄茎叶及根围土壤中的定殖动态,采用全基因组测序对其进行基因预测与功能注释。结果表明,在杜梨盆栽试验中,贝莱斯芽胞杆菌JS6-1喷施后,先呈下降趋势,3 d后呈上升趋势,7~45 d呈下降趋势,至喷施后45 d,菌株在茎叶上定殖密度为6.00×10^(5)CFU/g;灌根后,在土壤中定殖密度整体呈下降趋势,在7~15 d时略有回升,随后下降,至灌根45 d,菌株定殖密度为3.10×10^(5)CFU/g。在葡萄盆栽试验中,喷施和灌根后,菌株定殖量均呈下降趋势,施用45 d后,茎叶与根际土中菌株的定殖密度分别为3×10^(2)CFU/g和1.32×10^(4)CFU/g。全基因组测序结果显示,贝莱斯芽胞杆菌JS6-1全基因组长为4014208 bp,共编码3885个ORF,含有86个tRNA基因、27个rRNA、82个sRNA、76个串联重复序列;COG注释到氨基酸转运和代谢、编码多药转运蛋白等可能与抑菌能力有关的基因,GO数据库注释到跨膜转运蛋白活性基因有96个,KEGG数据库注释到有45个萜类和聚酮类化合物的代谢途径。综上,菌株JS6-1在室内盆栽杜梨和葡萄茎叶及根围土壤中均具有稳定的定殖能力,含有多种抗性基因,具有进一步研究和研发的价值。
The strain Bacillus velezensis JS6-1 obtained from the saline soil of Gashi County,Kashgar,Xinjiang and had good preventive effect on pear fire blight,grape mildew,grape powdery mildew and other crop diseases.JS6-1 was labeled by rifampicin in order to study its colonization characteristics.The whole genome was sequenced for gene prediction and functional annotation.The results showed that the colonization density of JS6-1 was 6.00×105 CFU/g in the stems and leaves of Pyrus betulifolia Bunge 3 d after being sprayed,and that was 6.00×10^(5) CFU/g 45 d after being sprayed.The colonization density decreased at first and increased 7 days after being irrigated in potting soil of Pyrus betulifolia Bunge,The colonization quantities reached 4.46×10^(5) CFU/g after 15 d and 3.10×10^(5) CFU/g 45d The colonization density of JS6-1 in the stem and leaves and in the soil were both decreasing.The colonization densities in the stem and leaves and in the inter-root soil were 3×10^(2) CFU/g and 1.32×10^(4) CFU/g,respectively,after 45 d of application.The whole genome sequencing results show that JS6-1 was 4014208 bp,encoding 3885 ORF,containing 86 tRNA genes,27 rRNA,82 sRNA,76 tandem repeats.Amino acid transport and metabolism,encoding multidrug transporters and other genes that may be related to bacteriostatic ability were annotated by COG.There were 96 transmembrane transporter active genes annotated by GO database;45 metabolic pathways of terpenoids and polyketides were annotated through the KEGG database.In conclusion,strain JS6-1 can colonizate in stem,leaf and root soil of potted Pyrus betulifolia Bunge and grape steadily,and contains a variety of resistance genes,which is valuable for further research and development.
作者
吴梓菲
林胜楠
史芳芳
王兴雯
王志慧
王宁
史应武
罗明
郭文超
包慧芳
WU Zifei;LIN Shengnan;SHI Fangfang;WANG Xingwen;WANG Zhihui;WANG Ning;SHI Yingwu;LUO Ming;GUO Wenchao;BAO Huifang(College of Agronomy,Xinjiang Agricultural University,Urumqi 830052,China;Xinjiang Key Laboratory of Special Environmental Microbiology/Institute of Microbiology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;The Institute of Agricultural Sciences of the 12th Division of Xinjiang Production and Construction Corps,Urumchi 830088,China;Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)
出处
《中国生物防治学报》
北大核心
2025年第2期335-346,共12页
Chinese Journal of Biological Control
基金
新疆自治区杰出青年基金(2022D01E63)
新疆农业科学院自主培育专项(xjnkycxzx-2022-003)
新疆兵团第十二师科技计划项目(SR202105)
农业科技创新稳定支持项目(xjnkywdzc-2023005)
新疆林果产业技术体系(XJLGCYJSTX05-2024-11)
新疆自治区重大专项(2023A0200604-03)。
关键词
贝莱斯芽胞杆菌
定殖动态
全基因组
基因注释
Bacillus velezensis
colonization dynamics
complete genome sequence
gene annotation
