摘要
采用硅胶、凝胶和半制备高效液相色谱等技术,从铁棒锤根的95%乙醇提取物中分离得到10个化合物,经UV、IR、HR-ESI-MS、NMR等多种波谱学方法鉴定其结构,分别为2个酚类化合物:4-[α-L-arabinofuranosyl-(1→6)-β-D-glucopyranosy-loxy]-3-methoxypropiophenone(1)、corchoionoside C(2),2个木脂素类化合物:(+)-oxabicyclooctalignan(3)、(-)-oxabicycloocta-lignan(4),5个二萜生物碱类化合物:benzoylaconine(5)、14-benzoyl-8-O-methylaconine(6)、(-)-(A-b)-14α-benzoyloxy-N-ethyl-13β,15α-dihydroxy-1α,6α,8β,16β,18-pentamethoxyaconitane(7)、smirnotine A(8)、aconitine(9),1个香豆素类化合物:scopoletin(10)。其中,化合物1为新的酚苷类化合物,化合物3、4、7、8、10为首次从铁棒锤中分离得到。采用脂多糖(LPS)诱导RAW264.7巨噬细胞的炎症模型测试了化合物的抗炎活性,化合物5、6、10能够抑制细胞中LPS诱导的一氧化氮(NO)的生成,其IC 50分别为(34.1±1.3)、(28.0±1.0)、(20.3±1.7)μmol·L-1。
Ten compounds were isolated from the 95%ethanol extract of the roots of Aconitum pendulum using silica gel chromatography,gel chromatography,and semi-preparative high-performance liquid chromatography.Their structures were identified by various spectroscopic methods,including UV,IR,HR-ESI-MS,and NMR.The compounds were identified as two phenolic compounds:4-[α-L-arabinofuranosyl-(1→6)-β-D-glucopyranosyloxy]-3-methoxypropiophenone(1)and corchoionoside C(2);two lignans:(+)-oxabicyclooctalignan(3)and(-)-oxabicyclooctalignan(4);five diterpenoid alkaloids:benzoylaconine(5),14-benzoyl-8-O-methylaconine(6),(-)-(A-b)-14α-benzoyloxy-N-ethyl-13β,15α-dihydroxy-1α,6α,8β,16β,18-pentamethoxyaconitane(7),smirnotine A(8),and aconitine(9);and one coumarin compound:scopoletin(10).Among them,compound 1 is a novel phenolic glycoside,while compounds 3,4,7,8,and 10 were isolated from A.pendulum for the first time.The anti-inflammatory activities of the compounds were evaluated using an LPS-induced RAW264.7 macrophage inflammation model.Compounds 5,6,and 10 inhibited LPS-induced nitric oxide(NO)production in the cells,with IC 50 values of(34.1±1.3),(28.0±1.0),and(20.3±1.7)μmol·L-1,respectively.
作者
董丹
陈澌祺
姜暖
余浩
马敏
DONG Dan;CHEN Si-qi;JIANG Nuan;YU Hao;MA Min(College of Pharmacy,Qinghai Minzu University,Xining 810007,China;Qinghai Provincial Key Laboratory of Phytoresource Chemistry Research on the Tibetan Plateau,Xining 810007,China;Modern Plateau Bio-pharmaceutical Industry College,Xining 810007,China)
出处
《中国中药杂志》
北大核心
2025年第22期6380-6386,共7页
China Journal of Chinese Materia Medica
基金
青海省科技厅自然科学基金青年项目(2022-ZJ-950Q)
青海民族大学2021年度高层次博士项目(2021XJG18)。
关键词
铁棒锤
化学成分
酚苷
抗炎活性
Aconitum pendulum
chemical constituents
phenolic glycoside
anti-inflammatory activity
