摘要
以黄皮(Clausena lansium)果核为原料,优化挥发油提取工艺,鉴定其成分并评估抗氧化活性。采用单因素试验和响应面法优化超声波辅助提取黄皮果核挥发油工艺参数,并利用气相色谱-质谱联用技术对黄皮果核挥发油进行成分分析,最后,考察其对1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl,DPPH)自由基、羟自由基及亚硝酸盐的清除能力,以评估抗氧化活性。结果表明:最优工艺参数为液料比8.5(mL:g)、超声温度41℃、超声时间27 min,在此提取条件下黄皮果核挥发油提取率达3.2%,共鉴定出26种成分;黄皮果核挥发油对DPPH自由基、羟自由基和亚硝酸盐的半数清除率(IC_(50))值分别为1.76、4.10×10^(-3)、9.06×10^(-3)mg/mL。黄皮果核挥发油对DPPH自由基的清除能力一般,但对羟自由基和亚硝酸盐的清除能力较强,表明其具有较强的抗氧化活性,对果实挥发油提取及功能分析具有参考价值。
The volatile oil extraction process of wampee(Clausena lansium)pit was optimized,and the volatile oil components were identified and the antioxidant activities were evaluated.Single-factor experiments and response surface method were used to optimize the parameters of ultrasonic-assisted extraction of volatile oil from wampee pit.The components of volatile oil from fruit pit were analyzed by gas chromatography-mass spectrometry(GC-MS).Finally,its antioxidant activity was evaluated by examining its scavenging ability against 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical,hydroxyl free radical and nitrite.The results showed that the optimal parameters were liquid to solid ratio of 8.5(mL:g),ultrasonic temperature of 41℃and ultrasonic time of 27 min.Under these conditions,the extraction rate of volatile oil reached 3.2%,and a total of 26 components were identified.The IC_(50) values of the DPPH radical,hydroxyl radical and nitrite clearance of the volatile oil were 1.76,4.10×10^(-3) and 9.06×10^(-3) mg/mL,respectively.The scavenging ability of the volatile oil on DPPH radical was normal,but the scavenging ability on hydroxyl radical and nitrite was outstanding,indicating that it had strong antioxidant activity,which had certain reference value for the extraction and functional analysis of volatile oil in fruits.
作者
刘金丰
萧允艺
邱研玲
罗剑斌
李颖
滕丽丽
LIU Jinfeng;XIAO Yunyi;QIU Yanling;LUO Jianbin;LI Ying;TENG Lili(Guangdong Provincial Key Laboratory for Green Agricultural Production and Intelligent Equipment,College of Biological and Food Engineering,Guangdong University of Petrochemical Technology,Maoming 525000,China;Guangdong Maoming Vocational College of Agriculture and Forestry Science and Technology,Maoming 525000,China)
出处
《食品科技》
北大核心
2025年第10期260-268,共9页
Food Science and Technology
基金
广东省普通高校特色创新项目(自然科学)(2023KTSCX090)
广东省普通高校重点领域专项(乡村振兴)项目(2020ZDZX1056)。
关键词
黄皮果核
挥发油
提取工艺
响应面法
wampee pit
volatile oil
extraction technology
response surface method
