摘要
建立花生仁及花生秸秆中吡唑醚菌酯残留量的检测方法,评价吡唑醚菌酯种子处理剂在花生生产应用中的膳食安全性。样品经乙腈匀浆提取、硫酸镁和乙酸钠盐析分层、C18和N-丙基乙二胺(PSA)净化,结合超高效液相色谱-串联质谱联用技术,对花生仁及花生秸秆中吡唑醚菌酯的残留水平进行测定;结合中国普通居民膳食结构数据,开展长期膳食摄入风险评估。结果表明,吡唑醚菌酯在0.005~0.200 mg/L浓度范围内线性关系良好(相关系数≥0.9999)。花生仁、花生秸秆中吡唑醚菌酯的平均添加回收率分别为73%~83%、66%~84%,相对标准偏差分别为1%~3%、3%~5%,方法检出限为1.4×10^(-5)~2.1×10^(-5) ng/μL,定量限为0.01 mg/kg,满足农作物农药残留检测技术要求。田间残留试验结果显示,两种施药剂量下,花生仁中吡唑醚菌酯的残留试验中值(STMR)与残留最大值(HR)均为0.010 mg/kg;花生秸秆中STMR为0.010 mg/kg,HR随施药剂量升高从0.053 mg/kg增加至0.073 mg/kg。膳食风险评估表明,在当前中国居民的膳食结构下长期膳食摄入风险概率(RQ)为38.7%。该检测方法操作便捷、灵敏度高、准确性好,可用于花生中吡唑醚菌酯残留监测;规范用药条件下,吡唑醚菌酯在花生中的残留对普通人群长期膳食摄入风险处于可接受水平。
An analytical method for determining pyraclostrobin residues in peanut kernels and peanut straw was established,and the dietary safety of pyraclostrobin seed treatment in peanut production was evaluated.The samples were homogenized and extracted with acetonitrile,followed by phase separation with magnesium sulfate and sodium acetate,and cleaned up using C18 and primary secondary amine(PSA).The residue levels of pyraclostrobin in peanut kernels and peanut straw were determined by UPLC-MS/MS.The long-term dietary intake risk was assessed by combining the residue data with the dietary structure of the Chinese general population.The results indicated that pyraclostrobin showed a good linear relationship in the concentration range of 0.005-0.200 mg/L(correlation coefficient≥0.9999).The average fortified recoveries of pyraclostrobin in peanut kernels and peanut straw were 73%-83%and 66%-84%,with relative standard deviations of 1%-3%and 3%-5%,respectively.The limit of detection and the limit of quantification were 1.4×10^(-5) ng/μL to 2.1×10^(-5) ng/μL and 0.01 mg/kg,respectively,which met the technical requirements for pesticide residue analysis in crops.The field residue trial results showed that the supervised trial median residue(STMR)and the highest residue(HR)of pyraclostrobin in peanut kernels were both 0.010 mg/kg at two application rates.The STMR in peanut straw was 0.010 mg/kg,while the HR increased from 0.053 mg/kg to 0.073 mg/kg with the increasing application rate.The dietary risk assessment revealed that the longterm dietary intake risk quotient(RQ)was 38.7%for the current dietary pattern of Chinese residents.The method was convenient,sensitive,and accurate,making it suitable for monitoring pyraclostrobin residues in peanuts.Under the recommended dosage,the residues of pyraclostrobin in peanuts posed an acceptable long-term dietary intake risk to the general population.
作者
陈鑫
吕昂
朱坤淼
刘丽
夏虹
刘军
CHEN Xin;LYU Ang;ZHU Kun-miao;LIU Li;XIA Hong;LIU Jun(Institute of Agricultural Quality Standards and Testing Technology Research,Hubei Academy of Agricultural Sciences/Hubei Key Laboratory of Nutrition Quality and Safety of of Agro-Products,Wuhan 430064,China)
出处
《湖北农业科学》
2025年第12期199-203,共5页
Hubei Agricultural Sciences
基金
国家农产品质量安全风险评估专项(14234073)。
