摘要
该研究以甘肃临夏地区泡菜为材料,采用溶钙圈法分离乳酸菌,采用形态学观察、生理生化实验及分子生物学技术鉴定其种属,并分析其耐受性、产酶能力、溶血性、药敏性和抑菌性等生物学特性。结果表明,共分离7株乳酸菌(编号分别为B2-3-1~B2-3-4、B2-3-6~B2-3-8),经鉴定,菌株B2-3-1为融合魏斯氏菌(Weissella confusa),菌株B2-3-2和B2-3-3为乳酸明串珠菌(Leuconostoc lactis),菌株B2-3-4为嗜热链球菌(Streptococcus thermophilus),菌株B2-3-6和B2-3-8为植物乳植杆菌(Lactiplantibacillus plantarum),菌株B2-3-7为副干酪乳杆菌(Lactobacillus paracasei);其中,菌株B2-3-8可在p H 2条件下生长,菌株B2-3-6和B2-3-8可在胆盐质量浓度0.7 g/L条件下生长,菌株B2-3-1、B2-3-6、B2-3-8可在12%NaCl条件下生长,菌株B2-3-3和B2-3-6可在80℃处理后生长;菌株B2-3-7具有产蛋白酶能力(D/d=2.18);菌株B2-3-8胞内β-半乳糖苷酶活性为36.72 U/m L;7株菌株对抗生素氯霉素、庆大霉素、左氧氟沙星均表现出低敏性;菌株B2-3-3、B2-3-7、B2-3-8对金黄色葡萄球菌(Staphylococcus aureus)和大肠杆菌(Escherichia coli)均有抑制作用;菌株B2-3-1、B2-3-4、B2-3-7、B2-3-8无溶血现象。综合考虑,菌株B2-3-8生物学特性优良。
Taking pickles from Linxia region,Gansu Province as the material,lactic acid bacteria were isolated by the calcium dissolution ring method.Their species were identified by morphological observation,physiological and biochemical experiments,and molecular biology techniques,and their biological characteristics such as tolerance,enzyme production capacity,hemolysis,drug sensitivity and antibacterial property were analyzed.The results showed that a total of 7 strains of lactic acid bacteria(numbered as B2-3-1 to B2-3-4,and B2-3-6 to B2-3-8)were isolated.After identification,strain B2-3-1 was Weissella confusa,strains B2-3-2 and B2-3-3 were Leuconostoc lactis,strain B2-3-4 was Streptococcus thermophilus,strains B2-3-6 and B2-3-8 were Lactiplantibacillus plantarum,and strain B2-3-7 was Lactobacillus paracasei.Among them,strain B2-3-8 could grow at pH 2,strains B2-3-6 and B2-3-8 could grow at bile salt mass concentration 0.7 g/L,strains B2-3-1,B2-3-6,and B2-3-8 could grow at NaCl 12%,strains B2-3-3 and B2-3-6 could grow at 80℃treatment,strain B2-3-7 possessed protease production capability(D/d=2.18),and the intracellularβ-galactosidase activity of strain B2-3-8 was 36.72 U/ml.All 7 strains exhibited low susceptibility to the antibiotics chloramphenicol,gentamicin,and levofloxacin.Strains B2-3-3,B2-3-7 and B2-3-8 exhibited inhibitory activity against both Staphylococcus aureus and Escherichia coli.Strains B2-3-1,B2-3-4,B2-3-7 and B2-3-8 showed no hemolysis.In conclusion,strain B2-3-8 demonstrated excellent biological characteristics.
作者
陈小龙
张挺峰
吴凤凤
杨林
康佳
李彩霞
CHEN Xiaolong;ZHANG Tingfeng;WU Fengfeng;YANG Lin;KANG Jia;LI Caixia(College of Life Science and Engineering,Hexi University,Zhangye 734000,China;Hexi Institute of Ecology,Zhangye 734000,China)
出处
《中国酿造》
北大核心
2025年第12期109-115,共7页
China Brewing
基金
甘肃省高等学校创新基金项目(2022A-123)
2024大学生创新创业训练计划项目(S202410740050)
河西学院第十四批大学生科技创新项目(2024-163)。
关键词
泡菜
乳酸菌
分离鉴定
耐受性
益生特性
安全性
pickles
lactic acid bacteria
isolation and identification
tolerance
probiotic property
safety
