摘要
研究旨在优化毛葡萄叶多糖超声辅助水提取法提取工艺,并评价毛葡萄叶多糖的抗氧化活性。在单因素试验基础上,以超声时间、超声温度、液料比、提取次数为自变量,以毛葡萄叶多糖得率为因变量,采用响应面法优化毛葡萄叶多糖超声辅助提取工艺,分别测定毛葡萄叶多糖对2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐[2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt,ABTS]自由基、1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picryl-hydrazyl radical,DPPH)自由基的清除能力和对铁离子的还原能力。结果表明:毛葡萄叶多糖超声辅助提取的最佳工艺为超声时间93 min、超声温度50℃、液料比123∶1(mL/g)、提取次数5次,多糖得率为12.91%,与模型预测值12.55%接近。毛葡萄叶多糖具有较好的抗氧化活性,其清除DPPH、ABTS自由基的半数清除浓度(half maximal inhibitory concentration,IC50)值分别为0.019、0.153 mg/mL,铁离子还原能力为3.57 mmol/g VC。
This experiment aimed to optimize the ultrasound-assisted water extraction process for polysac⁃charides from Vitis heyneana Roem.&Schult.leaves and evaluate their antioxidant activity.Based on single-factor experiments,the ultrasound-assisted extraction process was optimized using response surface methodology with ultrasound time,ultrasound temperature,liquid-to-solid ratio,and times of extraction cycles as independent variables,and the yield of Vitis heyneana Roem.&Schult.leaves polysaccharides as the dependent variable.The antioxidant activity of the polysaccharides was assessed by measuring their scavenging capacity against 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammo⁃nium salt(ABTS)and 1,1-Diphenyl-2-picrylhydrazyl radical(DPPH),as well as their ferric ion reducing power.The results showed that the opti⁃mal extraction conditions were an ultrasound time of 93 min,ultrasound temperature of 50℃,liquidto-solid ratio of 123∶1(mL/g),and 5 extraction cycles,yielding a polysaccharide yield of 12.91%,which was close to the model-predicted value of 12.55%.The polysaccharides exhibited strong antioxidant activity,with half maximal inhibitory concentration(IC50)values for DPPH and ABTS radical scavenging of 0.019 mg/mL and 0.153 mg/mL,respectively,and a ferric ion reducing power of 3.57 mmol/g VC.
作者
张勇
李榕娣
庄远杯
林大都
黄思涵
张声源
ZHANG Yong;LI Rongdi;ZHUANG Yuanbei;LIN Dadu;HUANG Sihan;ZHANG Shengyuan(Medical College,Jiaying University,Guangdong Meizhou 514031,China;Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas,Jiaying University,Guangdong Meizhou 514015,China)
出处
《饲料工业》
北大核心
2025年第24期163-171,共9页
Feed Industry
基金
2022年广东省基地嘉应学院客家研究院大客家平台研究团队项目[22KYKT03]
嘉应学院科研项目[2022KJY13]
梅州市医药卫生科研项目[2022-B-53]。
关键词
毛葡萄叶
多糖
响应面法
抗氧化活性
提取工艺
Vitis heyneana Roem.&Schult.leaves
polysaccharides
response surface
antioxidant activity
extraction process
