摘要
目的探索氢气(hydrogen,H_(2))抑制单采血小板活化和凋亡的作用研究。方法10份单采血小板,每份平均装入16小袋中,随机分为H_(2)组和对照组,H_(2)组加入H_(2)至饱和,并每天补充H_(2)。(22±2)℃振荡保存8天,每天检测血小板计数,流式检测CD62P、PAC-1及凋亡的百分比,Western blotting检测Caspase-3、Caspase-9、Bax、Bcl-2蛋白的表达水平。结果血小板计数在保存期间(1~8天)的差异均无统计学意义。但流式实验的凋亡指标从第5天开始,H_(2)组的比例比对照组低(20.29±4.32 vs 33.06±8.01,P<0.001);活化指标CD62P从第6天开始,H_(2)组比对照组低(24.94±5.53 vs 47.14±10.41,P<0.001),PAC-1从第6天开始,H_(2)组比对照组低(14.89±5.98 vs 19.41±5.17,P<0.05),差异有统计学意义。Western blotting结果显示从第6天开始,H_(2)组cleaved Caspase-9和cleaved Caspase-3含量表达比对照组低(P<0.001),第7天开始,H_(2)组Bax含量表达比对照组低(P<0.001),Bcl-2比对照组高(P<0.05),Bax/Bcl-2的比值比对照组低,差异有统计学意义(P<0.001)。结论H_(2)可以抑制单采血小板活化和凋亡,一定程度上改善保存质量。
Objective To explore the effect and mechanism of hydrogen(H_(2))on apheresis platelets activation and apoptosis.Methods 10 apheresis platelets were divided equally into 16 small bags and randomly divided into an H_(2)group and a control group.The H_(2)group was added with hydrogen to saturation and supplemented with hydrogen daily.All samples were stored under agitation at(22±2)℃ for 8 days,the platelet count was tested,and the CD62P,PAC-1,apoptosis were detected by flow cytometry daily.The Caspase-3,Caspase-9,Bax,and Bcl-2 proteins were detected by Western blotting.Results There was no significant difference in platelet count during the preservation period(1~8 d).From the fifth day,the apoptosis rate in H_(2)group was significantly lower than that in the control group(20.29±4.32 vs 33.06±8.01,P<0.001).From the sixth day,the expression of CD62P in H_(2)group was lower than that in the control group(24.94±5.53 vs 47.14±10.41,P<0.001),and the expression of PAC-1 in H_(2)group was lower than that in the control group(14.89±5.98 vs 19.41±5.17,P<0.05).Western blotting results showed that from the sixth day,the expression of cleaved Caspase-9 and cleaved Caspase-3 in the H_(2)group was lower than that in the control group(P<0.001).From the seventh day,the expression of Bax in the H_(2)group was lower than that in the control group(P<0.001),while Bcl-2 was higher than that in the control group(P<0.05),the ratio of Bax/Bcl-2 was lower than that of the control group.Conclusion Hydrogen can reduce platelet activation and apoptosis,improve the quality of apheresis platelet storage.
作者
李津杞
秦爱华
任瑜菲
唐河山
陈翠敏
钱宝华
臧艳
LI Jinqi;QIN Aihua;REN Yufei;TANG Heshan;CHEN Cuimin;QIAN Baohua;ZANG Yan(Department of Transfusion Medicine,First Affiliated Hospital of Naval Medical University,Shanghai 200433;Clinical Research Center of the First Affiliated Hospital of Naval Medical University,Shanghai 200433)
出处
《临床输血与检验》
2025年第6期775-782,共8页
Journal of Clinical Transfusion and Laboratory Medicine
基金
海军军医大学第一附属医院“234学科攀峰计划”(No.2020YXK036)资助。
关键词
氢气
血小板保存损伤
活化
凋亡
Hydrogen
Platelet storage lesion
Activation
Apoptosis
