摘要
旨在为脂溶性生物活性物质的包埋递送提供参考,以大豆分离蛋白(SPI)-葡聚糖共轭物(SDC)为乳化剂和稳定剂,采用两步法运用高压微射流制备Pickering乳液(SDC-PEs),通过单因素实验研究了均质压力、均质次数、SDC添加量对乳液粒径和多分散指数(PDI)的影响,并采用响应面实验对SDC-PEs的制备条件进行了优化。另外,对最佳工艺条件下制备的SDC-PEs性质进行了分析。结果表明:SDC-PEs的最佳制备工艺条件为均质压力140 MPa、均质次数6次、SDC添加量2.13%、油相体积分数10%,在此条件下SDC-PEs的平均粒径为210.33 nm, PDI小于0.20;SDC-PEs表现出良好的pH稳定性、抗离子强度稳定性、温度稳定性和储存稳定性,且优于以SPI稳定的Pickering乳液(SPI-PEs)。综上,通过添加SDC可以制备出稳定性良好的Pickering乳液。
To provide a reference for the encapsulation and delivery of lipophilic bioactive substances,Pickering emulsions(SDC-PEs)was prepared by using a two-step method of high-pressure microfluidization process with soybean protein isolate(SPI)-dextran conjugate(SDC)as emulsifier and stabilizer.Single-factor experiments were conducted to investigate the effects of homogenization pressure,homogenization times,and SDC dosage on emulsion particle size and polydispersity index(PDI).The preparation conditions of SDC-PEs were further optimized using response surface methodology.Additionally,the properties of SDC-PEs prepared under the optimal conditions were analyzed.The results showed that the optimal preparation parameters of SDC-PEs were as follows:homogenization pressure 140 MPa,homogenization times 6,SDC dosage 2.13%,and volume fraction of oil phase 10%.Under these conditions,SDC-PEs exhibited an average particle size of 210.33 nm with a PDI below 0.20.The SDC-PEs showed excellent stability against pH,ionic strength,temperature,and storage,outperforming SPI-stabilized Pickering emulsions(SPI-PEs).In conclusion,Pickering emulsions with good stability can be prepared by adding SDC.
作者
李顺发
邓雨冰
胡醇
王良俊
张清
LI Shunfa;DENG Yubing;HU Chun;WANG Liangjun;ZHANG Qing(College of Food Science,Sichuan Agricultural University,Ya'an 625014,Sichuan,China;Rural Revitalization and Development Service Center of Meishan Tianfu New District,Meishan 620000,Sichuan,China)
出处
《中国油脂》
北大核心
2025年第12期41-48,共8页
China Oils and Fats
基金
四川天府新区眉山管委员会科技合作项目(2322339004)
雅安市-四川农业大学市校合作项目(23ZDYF0003)。
