摘要
以江西铅山红芽芋的大田球茎保种苗、愈伤组织再分化苗、3年室内激素继代培养苗和5年室内激素继代培养苗为对照,采用SRAP分子标记技术对其传统培养法茎尖脱毒苗、玻璃化冷冻疗法茎尖脱毒苗、包埋玻璃化冷冻疗法茎尖脱毒苗、包埋脱水冷冻疗法茎尖脱毒苗和小滴玻璃化冷冻疗法茎尖脱毒苗的遗传稳定性进行了检测。结果表明:江西铅山红芽芋SRAP较佳的12对引物为P13、P19、P22、P24、P26、P34、P35、P36、P41、P44、P50和P52;以这12对引物对9个江西铅山红芽芋样品进行PCR扩增,共得到773个总位点和582个多态位点,平均多态百分比为74.62%;多态位点的范围为22~65个;不同引物检测到的观察等位基因数(N_(a))范围为1.5946~1.8475,有效等位基因数(N_(e))范围为1.3117~1.4646,Nei’s基因多样性指数(H)范围为0.1886~0.2707,Shannon’s信息指数(I)范围为0.2882~0.4109。江西铅山红芽芋9个样品的遗传相似系数为0.5964~0.8797,遗传相似系数均超过0.5,说明江西铅山红芽芋9个样品的遗传差异较小。聚类分析结果表明,江西铅山红芽芋的传统培养法茎尖脱毒、包埋脱水冷冻疗法茎尖脱毒、包埋玻璃化冷冻疗法茎尖脱毒和小滴玻璃化冷冻疗法茎尖脱毒不会造成江西铅山红芽芋的遗传变异,但愈伤组织再分化、室内激素继代培养和玻璃化法冷冻疗法茎尖脱毒可能会造成江西铅山红芽芋的遗传变异。
The genetic stability of shoot-tip conventional culture virus-free plantlets,shoot-tip virus-free plantlets by vitrification cryotherapy,shoot-tip virus-free plantlets by encapsulation vitrification cryotherapy,shoot-tip virus-free plantlets by encapsulation dehydration cryotherapy,and shoot-tip virus-free plantlets by droplet vitrification cryotherapy was tested using SRAP molecular marker technology,with field planted seedlings,callus regenerated plantlets,3-year plantlets subcultured at room temperature,and 5-year plantlets subcultured at room temperature of Jiangxi Yanshan red sprout taro as controls.The results showed that the optimal 12 pairs of primers for SRAP in Jiangxi Yanshan red sprout taro were P13,P19,P22,P24,P26,P34,P35,P36,P41,P44,P50 and P52.The 12 pairs of primers were used for PCR amplification of 9 samples of Jiangxi Yanshan red sprout taro,and a total of 773 loci were obtained,including 582 polymorphic loci,with a polymorphic percentage of 74.62%.The total number of loci amplified by different primers varied,with a range of 22~65 polymorphic loci.The range of observed allele numbers(N_(a))detected by different primers was 1.5946~1.8475,the range of effective allele numbers(N_(e))was 1.3117~1.4646,the range of Nei’s gene diversity index(H)was 0.1886~0.2707,and the range of Shannon’s information index(I)was 0.2882~0.4109.The genetic similarity coefficients of the 9 samples of Jiangxi Yanshan red sprout taro were 0.5964~0.8797,all of which exceed 0.5,indicating that the genetic differences among the 9 samples of Jiangxi Yanshan red sprout taro were relatively small.The clustering analysis results show that shoot-tip conventional culture virus-free,shoot-tip virus-free by encapsulation dehydration cryotherapy,shoot-tip virus-free by encapsulation vitrification cryotherapy,and shoot-tip virus-free by droplet vitrification cryotherapy can not cause genetic variation in Jiangxi Yanshan red sprout taro.However,callus redifferentiation,normal temperature in vitro hormone subculture,and shoot-tip virus-free by vitrification cryotherapy may cause genetic variation in Jiangxi Yanshan red sprout taro.
作者
张艺欣
杜静怡
何凌婷
阿迪莱·吾马尔
哈丽米兰·艾合买提
张芝瑶
闵凌瑞
查淑丽
ZHANG Yi-xin;DU Jing-yi;HE Ling-ting;Adile Wumar;Harimillan Ahemaiti;ZHANG Zhi-yao;MIN Ling-rui;ZHA Shu-li(College of Life Sciences,Shangrao Normal University,Shangrao 334001,China)
出处
《江西农业学报》
2025年第12期69-78,共10页
Acta Agriculturae Jiangxi
基金
国家自然科学基金项目(31860084、32060092)。
关键词
江西铅山红芽芋
脱毒苗
遗传稳定性
SRAP检测
Jiangxi Yanshan red sprout taro
Virus-free plantlet
Genetic stability
SRAP detection
