摘要
随着人们对健康关注度的持续攀升,天然药用植物资源的深度开发与利用已成为当下科研领域的热点方向。在此背景下,对金花葵药用价值的开发研究具有重要的意义。金花葵作为一种颇具潜力的药用植物,富含黄酮醇类化合物。F3H基因作为黄酮醇生物合成途径中的关键基因,其编码的酶负责催化黄酮醇合成的关键步骤。因此,对F3H基因的深入研究有助于揭示植物次生代谢调控网络以及植物适应环境变化的分子策略。该试验以金花葵为材料,成功克隆出Am F3H基因,其全长1101bp,编码366个氨基酸。对Am F3H基因进行生物信息学分析、表达分析及功能验证,结果表明:Am F3H可以促进金花葵黄酮醇的合成,是金花葵黄酮醇合成途径中的关键酶基因。该研究通过转录组分析及表达模式分析,对Am F3H上游潜在的MYB转录因子进行了筛选,为后续金花葵黄酮醇合成调控网络的构建奠定了基础。
With the growing public focus on health,the in-depth development and utilization of natural medicinal plant resources have become a research hotspot in the scientific community.In this context,studies on exploiting the medicinal value of Abelmoschus manihot L.are of significant research significance.As a promising medicinal plant,Abelmoschus manihot L.is rich in flavonols.The F3H,a key gene in the flavonol biosynthesis pathway,encodes an enzyme that catalyzes a critical step.Therefore,in-depth research on the F3H gene is conducive to revealing the regulatory network of plant secondary metabolism and the molecular strategies by which plants adapt to environmental changes.In this study,using Abelmoschus manihot L.as experimental material,the AmF3H gene was successfully cloned,with a full length of 1101bp encoding 366 amino acids.Bioinformatics analysis,expression analysis,and functional verification of the AmF3H gene demonstrated that AmF3H can promote flavonol synthesis in Abelmoschus manihot L.and is a key enzyme gene in its flavonol synthesis pathway.Through transcriptome analysis and expression pattern analysis,potential MYB transcription factors upstream of AmF3H were screened,laying a foundation for subsequent construction of the flavonol synthesis regulatory network in Abelmoschus manihot L.
作者
陈斯文
董皓妍
楚洪涛
刘仲旭
安锐
王大运
曹丽
CHEN Siwen;DONG Haoyan;CHU Hongtao;LIU Zhongxu;AN Rui;WANG Dayun;CAO Li(College of Agriculture,Yanbian University,Yanji Jilin 133002,China)
出处
《延边大学农学学报》
2025年第4期43-53,共11页
Journal of Agricultural Science Yanbian University
基金
国家自然科学基金项目(31960619)
吉林省教育厅教研课题(JJKH20180887KJ)。
关键词
F3H基因
黄酮醇
金花葵
基因克隆
功能分析
F3H gene
flavonol
Abelmoschus manihot L.
gene cloning
functional analysis
