摘要
目的:基于网络药理学探讨万年蒿抑制肥胖的作用机制。方法:查阅文献,找到万年蒿的有效活性成分,通过Swiss target prediction预测有效活性成分对应靶点;从Genecards和Disgenet数据库中找到关于肥胖的所有基因,并和有效成分对应的靶点取交集,制作Venny图;运用Cytoscape 3.9.1绘制药物-有效活性成分-靶点网络图,通过String数据库,对交集靶点进行蛋白与蛋白交互作用网络构建;利用David数据库进行基因本体论(GO)富集分析和京都基因和基因组百科全书(KEGG)富集分析。结果:共找到83个万年蒿的有效活性成分。万年蒿对应靶标与肥胖对应靶标的交集共342个。其中,关键的有效成分为木犀草素、芫花黄素、高车前素、山柰酚、木犀草素-5,3'-二甲醚和苜蓿素;关键的靶点有甘油醛-3-磷酸脱氢酶(GAPDH)、蛋白激酶B(AKT1)、肿瘤坏死因子(TNF)和白细胞介素-6(IL-6)。GO富集分析结果显示,与生物过程相关的主要有对外源性刺激的反应、核糖核酸(RNA)聚合酶Ⅱ启动子转录的正调控、蛋白磷酸化、基因表达的正调控和凋亡过程的负调控;与分子功能相关的主要有蛋白质结合、酶结合、丝氨酸/苏氨酸/酪氨酸激酶活性、RNA聚合酶Ⅱ转录因子活性、配体激活序列特异性DNA结合及相同蛋白结合;与细胞组成相关的主要分布在质膜、质膜的组成部分、细胞质、胞质溶胶和核质。KEGG富集分析结果显示,主要富集在癌症的途径、糖尿病并发症中的高级糖基化终末产物-受体(AGE-RAGE)信号通路、脂质和动脉粥样硬化以及化学致癌-受体激活。结论:万年蒿是通过多靶点、多通道来发挥抑制肥胖的作用。
Objective:To explore the mechanism of Artemisia sacrorum in inhibiting obesity based on network pharmacology.Methods:The literature was reviewed to identify the active ingredients of Artemisia sacrorum.The targets corresponding to these active ingredients were predicted using Swiss Target Prediction.All genes related to obesity were retrieved from the Genecards and Disgenet databases and intersected with the targets of the active ingredients to create a Venny diagram.Cytoscape 3.9.1 was used to construct a drug-active ingredient-target network.The String database was employed to build a protein-protein interaction(PPI)network for the intersecting targets.The David database was utilized for Gene Ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.Results:A total of 83 active ingredients of Artemisia sacrorum were identified.The intersection of targets corresponding to Artemisia sacrorum and those related to obesity yielded 342 common targets.Key active ingredients included luteolin,genkwanin,hispidulin,kaempferol,5,3'-dimethoxy luteolin,and tricin.Key targets included Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH),Protein Kinase B(AKT1),Tumor Necrosis Factor(TNF),and Interleukin-6(IL-6).GO enrichment analysis results indicated that the primary biological processes involved were response to external stimulus,positive regulation of transcription from RNA polymerase II promoter,protein phosphorylation,positive regulation of gene expression,and negative regulation of apoptotic process.The main molecular functions included protein binding,enzyme binding,serine/threonine/tyrosine kinase activity,RNA polymerase II transcription factor activity,ligand-activated sequence-specific DNA binding,and identical protein binding.The cellular components were mainly distributed in the plasma membrane,integral component of plasma membrane,cytoplasm,cytosol,and nucleoplasm.KEGG enrichment analysis results showed significant enrichment in pathways in cancer,the AGE-RAGE signaling pathway in diabetic complications,lipid and atherosclerosis,and chemical carcinogenesis-receptor activation.Conclusion:Artemisia sacrorum exerts its anti-obesity effects through multiple targets and pathways.
作者
于鹏
蒋岚
许光华
YU Peng;JANG Lan;XU Guanghua(College of Pharmacy,Yanbian University,Yanji 133002,China)
出处
《延边大学医学学报》
2025年第12期116-123,共8页
Journal of Medical Science Yanbian University
基金
吉林省自然科学基金项目(编号:YDZJ202201ZYTS229)。
关键词
万年蒿
网络药理学
肥胖
Artemisia sacrorum
network pharmacology
obesity
