摘要
目的 观察“扶正固肾”针灸穴方对顺铂(DDP)肾损伤小鼠肾脏组织中p-JNK、p-ERK和p-p38的影响,探究针灸改善化疗致肾损伤的作用机制。方法 将40只小鼠随机划分为4组,即空白组、模型组、针刺组与艾灸组,每组各10只。除空白组外,其余3组小鼠均采用腹腔注射10 mg/kg顺铂(DDP)的方式进行造模,造模过程在24 h内完成;空白组则腹腔注射等量的0.9%氯化钠溶液。针刺组与艾灸组均选取“大椎”“肝俞”“肾俞”“足三里”,分别进行针刺与艾灸治疗,其余两组仅陪同固定,1次/d,连续5 d。禁食1 d后取材,通过HE染色法对小鼠肾脏组织的病理改变进行观察,ELISA法和Western Blot法检测肾脏组织中磷酸化c-Jun氨基末端激酶(p-JNK)、磷酸化细胞外信号调节蛋白激酶(p-ERK)和磷酸化丝裂原活化蛋白激酶(p-p38)的含量和蛋白表达。结果 相比于空白组,模型组肾脏组织中p-JNK、p-ERK和p-p38含量、蛋白表达均增多(P<0.05);相比于模型组,针刺组和艾灸组肾脏组织中p-JNK、p-ERK和p-p38含量、蛋白表达均减少(P<0.05)。结论“扶正固肾”针灸能够改善化疗所致肾损伤,其作用机制或许与下调JNK、ERK、p38的磷酸化水平存在关联。
Objective To observe the effects of“strengthening healthy qi and consolidating kidney”acupuncture-moxibustion acupoint prescription on p-JNK,p-ERK and p-p38 in kidney tissue of mice with cisplatin(DDP)kidney injury,and to explore the mechanism of acupuncture and moxibustion to improve chemotherapy-induced kidney injury.Methods Forty clean male Kunming mice were divided into four groups(n=10 each):the blank,model,acupuncture,and moxibustion groups.Except blank group,the other three groups were modeled by intraperitoneal injection of DDP(10 mg/kg).The modeling was completed within 24 hours,and the blank group was injected with the same dose of 0.9%sodium chloride solution.“Dazhui(GV 14)”,“Ganshu(BL 18)”,“Shenshu(BL 23)”and“Zusanli(ST 36)”were selected in acupuncture group and moxibustion group,and acupuncture and moxibustion were administered respectively,and the other two groups were only restrained without treatment once a day for 5 consecutive days.After fasting for 1 day,samples were collected.Hematoxylin-eosin(HE)staining was used to observe pathological changes in the mouse kidney tissues.ELISA and Western blot(WB)measured contents and protein expression of phosphorylated c-Jun amino-terminal kinase(p-JNK),phosphorylated extracellular signal-regulated protein kinase(p-ERK)and phosphorylated p38 mitogen-activated protein kinase(p-p38)in renal tissues.Results Compared with the blank group,contents and protein expressions of p-JNK,p-ERK and p-p38 in kidney tissues of model group were increased(P<0.05).Compared with model group,the contents and protein expressions of p-JNK,p-ERK and p-p38 in kidney tissues of acupuncture group and moxibustion group were decreased(P<0.05).Conclusion Acupuncture and moxibustion can improve kidney injury induced by DDP,and its mechanism may be related to downregulating JNK,ERK and p38 phosphorylation levels.
作者
于冬冬
韩乐
刘昭坤
杜雪源
路玫
王晴晴
王瑞泽
胡凯迪
康书福
YU Dongdong;HAN Le;LIU Zhaokun;DU Xueyuan;LU Mei;WANG Qingqing;Wang Ruize;Hu Kaidi;KANG Shufu(Henan University of Chinese Medicine,Zhengzhou 450046,China;Third Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450008,China)
出处
《时珍国医国药》
北大核心
2025年第24期4778-4782,共5页
JOURNAL OF LI-SHIZHEN TRADITIONAL CHINESE MEDICINE
基金
国家自然科学基金(81804196,82405594)
河南省重点研发与推广专项(科技攻关)(232102311195)
河南省自然科学基金面上项目(252300423168)
河南省省级优质国际化课程(HZC2025KC17)。
关键词
针灸
顺铂
肾损伤
MAPK信号通路
Acupuncture and moxibustion
Cisplatin
Kidney injury
MAPK signaling pathway
