摘要
为了解湖南省湘西土家族苗族自治州(简称“湘西州”)猪群中猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)、猪伪狂犬病病毒(PRV)及猪圆环病毒2型(PCV2)等4种常见猪源病毒的流行情况,2021—2024年采用分层抽样策略,采集无害化处理厂病死猪样品345份(淋巴结275份、耳尖70份)及屠宰场屠宰生猪淋巴结样品1168份,通过实时荧光PCR法检测病原核酸。结果显示:无害化处理厂病死猪中,PCV2、PRRSV、CSFV、PRV的阳性检出率分别为39.13%、16.23%、5.22%、1.45%,屠宰场屠宰生猪分别为15.41%、3.34%、0、2.31%;混合感染以PCV2+PRRSV为主,其中无害化处理厂病死猪中这2种病毒的混合感染检出率为10.72%,屠宰场屠宰生猪为2.65%。结果表明,湖南省湘西州猪群中PCV2和PRRSV流行可能较为严重,CSFV、PRV的潜在流行风险依然存在,病毒混合感染现象较突出。结果提示:应重点加强对PCV2和PRRSV的控制,对CSFV和PRV也不容忽视;规模猪场应加强免疫和种源管理,做好生物安全防控,主管机构应加强监测和检疫监管,防止病原侵入和传播。
In order to identify the prevalence of 4 common viruses including classical swine fever virus(CSFV),porcine reproductive and respiratory syndrome virus(PRRSV),pseudorabies virus(PRV)and porcine circovirus type 2(PCV2)in pig herds in Xiangxi Tujia and Miao Autonomous Prefecture of Hunan Province,345 samples(including 275 lymph nodes and 70 ear tips)were collected from dead pigs in rendering plants,and 1168 lymph node samples from slaughtered pigs in slaughterhouses using a stratifi ed sampling strategy from 2021 to 2024,to detect pathogen nucleic acids using real-time fl uorescent PCR.The results showed that the positive detection rates for PCV2,PRRSV,CSFV and PRV were 39.13%,16.23%,5.22%and 1.45%respectively in the dead pigs in rendering plants,and 15.41%,3.34%,0 and 2.31%respectively in the slaughtered pigs in slaughterhouses;and co-infection,primarily PCV2+PRRSV,was prominent,with the detection rate of 10.72%in rendering plants and 2.65%in slaughterhouses.In conclusion,PCV2 and PRRSV were severely prevalent in pig herds in the region,with potential risk of other two pathogens,especially viral co-infection.Therefore,other pathogens should not be neglected,although PCV2 and PRRSV should be controlled as a priority;vaccination and genetic management should be improved to ensure biosafety prevention and control on intensive farms,monitoring and quarantine supervision should be strictly implemented by competent authorities to prevent any introduction or transmission of pathogens.
作者
王锐
吴国海
向导
向贤宜
王鹏
杨丰川
王成
Wang Rui;Wu Guohai;Xiang Dao;Xiang Xianyi;Wang Peng;Yang Fengchuan;Wang Cheng(Animal Husbandry and Aquatic Products Aff airs Center of Xiangxi Tujia and Miao Autonomous Prefecture,Jishou 416000,Hunan,China;Animal Husbandry and Aquatic Products Aff airs Center of Guzhang County,Guzhang 416300,Hunan,China)
出处
《中国动物检疫》
2025年第12期22-27,共6页
China Animal Health Inspection
