摘要
碳源不足是导致低碳氮比(C/N)污水脱氮效果不佳的重要原因。以5种农业废弃物发酵液为碳源,考察其对低C/N污水的脱氮效果。结果表明:葡萄糖处理组(对照)总氮(TN)去除率最高,达到92.83%,其次是玉米秸秆(88.76%)、脐橙皮(88.55%)、油菜秸秆(83.60%)和水稻秸秆(82.47%),甘蔗渣(42.95%)最低。水稻秸秆[5.41mg/(g·h)]和玉米秸秆[5.78 mg/(g·h)]在外源反硝化阶段(EXD)反硝化速率(V_(DN))表现最高。相较于葡萄糖处理组,5组农业废弃物发酵液均显著提高了细菌Alpha多样性。进一步对反硝化功能菌属(20个)分析发现,相较于葡萄糖处理组,农业废弃物发酵液显著提高了Candidatus_Competibacter和Hydrogenophaga等6个功能菌属的相对丰度,而Dechloromonas与Comamonas则相反。此外,农业废弃物发酵液处理组(nirK+nirS)/nosZ均显著低于葡萄糖(P<0.05),这意味着农业废弃物发酵液相较于葡萄糖更有利于降低一氧化二氮(N_(2)O)与氮气(N_(2))的产物比。农业废弃物发酵液碳源(除甘蔗渣外)不仅能够有效提升低C/N污水的脱氮效果,同时有利于抑制反硝化过程中N2O的产生,可作为绿色、高效的脱氮处理碳源。
The scarcity of carbon source is a significant factor contributing to the poor denitrification efficiency of wastewater with low carbon to nitrogen(C/N)ratio.In this study,five types of agricultural waste fermentation broths were employed as carbon sources to assess their denitrification effectiveness on low C/N wastewater.The results indi⁃cated that the glucose treatment group exhibited the highest total nitrogen(TN)removal rate of 92.83%,followed by corn straw(88.76%),navel orange peel(88.55%),rapeseed straw(83.60%),and rice straw(82.47%).Bagasse had the lowest removal rate of 42.95%.Rice straw〔5.41 mg/(g·h)〕and corn straw〔5.78 mg/(g·h)〕exhibited the high⁃est denitrification rate(VDN)during the exogenous denitrification stage(EXD).Compared to the glucose treatment group,all five groups of agricultural waste fermentation broths significantly enhanced bacterial alpha diversity.Upon further analysis of 20 denitrification functional genera,it was observed that the fermentation broths of agricultural waste significantly increased the relative abundance of six genera,including Candidatus_Competibacter and Hydrogenophaga,compared to the glucose treatment group.However,the trends were reversed for Dechloromonas and Comamonas.In addition,the ratio of(nirK+nirS/nosZ)in the agricultural waste fermentation broth treatment group was significantly lower than that of glucose(P<0.05),which indicated that the agricultural waste fermentation liquid was more conducive to reducing the product ratio of nitrous oxide(N_(2)O)to nitrogen(N_(2))than glucose.In conclu⁃sion,the carbon source of agricultural waste fermentation liquid(except bagasse)could not only effectively enhance the denitrification effect of low C/N wastewater,but also inhibited the production of N_(2)O in the denitrification pro⁃cess,which could be used as a green and efficient carbon source for denitrification.
作者
段亦鹏
吴永明
张兴尔
汤爱萍
邓觅
刘亚军
DUAN Yipeng;WU Yongming;ZHANG Xing'er;TANG Aiping;DENG Mi;LIU Yajun(College of Environmental and Chemical Engineering,Nanchang Hangkong University,Nanchang 330063,China;Institute of Microbiology,Jiangxi Academy of Sciences,Nanchang 330096,China;Jiangxi Provincial Key Laboratory for Prevention and Control of Industrial Water Pollution,Nanchang 330096,China;Wuhan Sentai Environmental Protection Co.,Ltd.,Wuhan 430070,China)
出处
《工业水处理》
北大核心
2025年第12期129-136,共8页
Industrial Water Treatment
基金
江西省科技计划项目(20232BCJ22047)
江西省科学院省级科研项目经费包干制试点示范项目(2023YRCS001,2023YSBG22012,2023YSBG21004)
江西省科学院引进博士项目(2023YYB01)。
关键词
反硝化
碳源
农业废弃物
微生物
denitrification
carbon source
agricultural waste
microorganism
