摘要
目的对青海湖地区国家一级保护动物普氏原羚(Procapra przewalskii)中隐孢子虫(Cryptosporidium)的感染流行情况展开调查。方法基于隐孢子虫18S rRNA基因的巢式PCR方法对采集到的普氏原羚新鲜粪便样品基因组DNA进行扩增,以检测隐孢子虫感染率,并基于gp60基因片段对隐孢子虫阳性样本进行亚型分析。结果普氏原羚中隐孢子虫的感染率为3.67%(16/436),共检测到肖氏隐孢子虫(C.xiaoi)和泛在隐孢子虫(C.ubiquitum)2种隐孢子虫,其中C.xiaoi为优势虫株,占阳性样品的68.75%(11/16)。对C.ubiquitum阳性样本进行亚型分析,鉴定出其为XIIa型。结论本次研究首次证实普氏原羚中存在隐孢子虫感染,并且存在能够感染人的XIIa亚型C.ubiquitum,具有较大的人兽共患病传播风险。
This study was aimed at determining the prevalence of Cryptosporidium spp.in Procapra przewalskii,a nationally protected first-class species in China,and evaluated the zoonotic potential of the isolates.Fresh fecal specimens(n=436)were collected from P.przewalskii in the Qinghai Lake region.Nested PCR targeting the conserved region of the Cryptosporidium 18S rRNA gene was used for primary detection.Positive samples were subtyped with the gp60 gene fragment.An overall infection rate of 3.67%(16/436)was observed.Molecular characterization identified two species:Cryptosporidium xiaoi(68.75%,11/16)and C.ubiquitum(31.25%,5/16).C.ubiquitum positive samples were subtyped as XIIa.This study provides the first confirmation of Cryptosporidium infection in P.przewalskii.The presence of C.ubiquitum subtype XIIa,which can infect humans,indicates high zoonotic risk.Therefore,further surveillance and management are needed to safeguard the health of both P.przewalskii and humans.
作者
赵心愿
张迪迪
张学勇
马利青
李秀萍
王光华
简莹娜
ZHAO Xinyuan;ZHANG Didi;ZHANG Xueyong;MA Liqing;LI Xiuping;WANG Guanghua;JIAN Yingna(College of Agriculture and Animal Husbandry,College of Animal Husbandry and Veterinary Science,Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control,Qinghai University,Xining 810016,China)
出处
《中国人兽共患病学报》
北大核心
2025年第11期1179-1184,共6页
Chinese Journal of Zoonoses
基金
国家自然科学基金(No.32060804)
青海省“昆仑英才・高端创新创业人才”拔尖人才项目(No.2024)。
关键词
普氏原羚
隐孢子虫
感染率
人兽共患
Procapra przewalskii
Cryptosporidium
infection rate
zoonotic
