摘要
目的探讨骨碎补总黄酮通过破骨前体/成骨细胞“双向”调控H血管偶联成骨促进诱导膜技术二期骨重建的分子机制。方法将大鼠按体重分层后随机分为每组12只的空白组、模型组和骨醉补总黄酮组(TFRD组)。空白组大鼠制作4 mm的股骨骨缺损模型,而模型组和TFRD组建立与之相同尺寸的Masquelet诱导膜骨缺损模型。一期造模6周后,模型组和TFRD组将大鼠尾骨颗粒植入诱导膜内,空白组植入到骨缺损处,TFRD组予157.5 mg/(kg·d)的强骨胶囊灌胃,模型组和空白组予等量生理盐水灌胃。二期植骨术后8周,使用X线和HE染色观察骨缺损区的骨重建情况和新生骨组织的形态学结构。通过免疫荧光双染色法观察新生骨组织中H型血管数量,免疫组化和Western-blot法检测破骨前体/成骨细胞相关细胞因子的表达情况。结果X线及HE染色显示,TFRD组截骨区骨重建水平较模型组和空白组更佳,局部有更多的新生骨组织(P<0.05);免疫荧光双染色法显示TFRD组H型血管数量较其余两组增多(P<0.05);免疫组化和Western-blot显示骨碎补总黄酮可上调新生骨组织中破骨前体/成骨细胞相关细胞因子的表达(P<0.05)。结论骨碎补总黄酮可通过破骨前体/成骨细胞“双向”调控H型血管偶联成骨,促进诱导膜技术骨重建。
Objective To investigate the molecular mechanism by which Total flavonoids of Rhizoma fortune promote bone reconstruction using the induced membrane technique through the“bidirectional”regulation of osteoclast precursor/osteoblasts coupling with H-type vessels.Methods Rats were stratified by weight and then randomly divided into three groups of 12 rats each:a blank group,a model group,and a traditional Chinese medicine group.The 4 mm femoral bone defect model was created in the control group,while the model and TCM groups established ainduced membrane model of equivalent size.Six weeks after the first-stage modeling,tail bone particles were implanted into the induced membrane.The TCM group was administered with a strong bone capsule at a dose of 157.5 mg/kg/day by gavage,while the model and control groups received an equal volume of normal saline.Eight weeks after the second-stage bone grafting,X-ray and HE staining were used to observe bone reconstruction and the morphological structure of new bone tissue in the defect area.H-type vessel count in the new bone tissue was observed by immunofluorescent double staining,and the expression of H-type vessel-related factors was detected by immunohistochemistry and Western blot.Results X-ray and HE staining showed improved bone regeneration in the TCM group versus the model and control groups,featuring increased new bone and vascular tissue within the defect site(P<0.05).Immunofluorescent double staining showed a significant increase in the number of H-type vessels in the TCM group(P<0.05).Immunohistochemistry and Western blot indicated that Total flavonoids of RhizomaDrynariae significantly upregulated the positive expression of H-type vessels in the new bone tissue(P<0.05).Conclusion The total flavonoids of rhizomadrynariae can regulate H-type vascular coupling osteogenesis through a“bidirectional”approach of osteoclasts/osteoblasts,promoting induced membrane technology for bone reconstruction.
作者
曾志奎
梁超轶
袁灵梅
熊伟
魏文强
邓文芳
唐国鑫
邱勋祥
ZENG Zhikui;LIANG Chaoyi;YUAN Lingmei;XIONG Wei;WEI Wenqiang;DENG Wenfang;TANG Guoxin;QIU Xunxiang(Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,China;Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053,China)
出处
《中国骨质疏松杂志》
北大核心
2025年第12期1727-1734,共8页
Chinese Journal of Osteoporosis
基金
国家自然科学基金(82460938,81960880)。
关键词
骨碎补总黄酮
H型血管
诱导膜
骨缺损
骨重建
total flavonoids of rhizomadrynariae
H-type vessels
induced membrane
bone defect
bone reconstruction
