摘要
目的:通过原位实验,探究槲皮素对人牙本质抗酸蚀性能的影响。方法:制备20个牙本质块(2 mm×2 mm×2 mm),随机分为两组,一组采用1.23×10^(4)μg/mL氟化钠,另一组使用300μg/mL的槲皮素。通过循环体外酸蚀-原位再矿化,以模拟口腔环境。牙本质经对应溶液处理后,10名志愿者每日佩戴含牙本质试件的牙合垫式牙托,期间进行4次体外循环酸蚀处理:柠檬酸(pH=2.45)浸泡5 min×4次,实验为期7 d。对样品进行酸蚀前后的表面显微硬度(surface microhardness,SMH)及表面轮廓的检测,并计算表面显微硬度变化量(SMH%)及表面丧失量。结果:所有志愿者均顺利完成实验流程。数据显示,槲皮素组的SMH%为(6.22±2.94)%,显著低于氟化钠组的(19.23±5.78)%(P<0.01)。槲皮素组的表面丧失量(0.97±0.34)μm也显著低于氟化钠组[(2.61±0.97)μm,P<0.01]。结论:在模拟日常口腔环境的条件下,短期(2 min)应用300μg/mL槲皮素溶液能够显著提升牙本质抗酸蚀性能,其效果优于传统高浓度[(1.23×10^(4))μg/mL]氟化钠溶液。
Objective:To investigate the effect of quercetin on the human dentin resistance to erosion on in-situ study.Methods:Twenty dentin blocks(2 mm×2 mm×2 mm)were prepared and randomly divided into two groups,which were treated with 1.23×10^(4)μg/mL sodium fluoride and 300μg/mL quercetin,respectively.The oral environment was simulated through the in-vitro acid and in-situ remineralization cycle.After treated with corresponding solution,dentin specimens were wore by ten volunteers in the customized intraoral appliance.Four cycles of acid etching were performed during this period:soaked in citric acid(pH=2.45)for 4 times with 5 min in each time.The experiment was lasted for 7 days.The SMH and surface profiles were detected before and after erosion by using the surface microhardness tester and contact profilometry,respectively.The SMH%and the surface loss were calculated.Results:In the in situ experiment,all volunteers completed the experiment process.The SMH%of quercetin group was(6.22±2.94)%,which was significantly lower than that of sodium fluoride group[(19.23±5.78)%,P<0.01].The surface loss in quercetin group[(0.97±0.34)μm]was significantly lower than that in sodium fluoride group[(2.61±0.97)μm,P<0.01].Conclusion:Based on the in situ experiment,the short-term(2 min)application of 300μg/mL quercetin solution can significantly improve the acid corrosion resistance of dentin,and the effect is better than the traditional high concentration[(1.23×10^(4))μg/mL]sodium fluoride solution.
作者
蒋能舞
黄玉霞
郑章龙
毕玮
JIANG Nengwu;HUANG Yuxia;ZHENG Zhanglong;BI Wei(Department of Prosthodontics,Affiliated Stomatological Hospital,Fujian Medical University,Fuzhou 350002,China;Department of Stomatology,Xiamen Branch,Zhongshan Hospital,Fudan University,Xiamen 361015,China;Department of Stomatology,Zhongshan Hospital,Fudan University,Shanghai 200032,China)
出处
《口腔医学研究》
北大核心
2025年第12期1055-1058,共4页
Journal of Oral Science Research
基金
福建省自然科学基金面上项目(编号:2022J011424)。
