摘要
目的:以结扎诱导的实验性牙周炎小鼠为模型,探讨延胡索酸(dimethyl fumarate,DMF)对实验性牙周炎小鼠牙槽骨丢失的影响。方法:分组如下:(1)正常小鼠+溶剂组:正常小鼠,每隔2 d灌胃0.8%羧甲基纤维素(carboxymethyl cellulose,CMC);(2)牙周炎小鼠+溶剂组:小鼠牙周结扎,每隔2 d龈沟注射大肠杆菌脂多糖(lipopolysaccharide,LPS)并灌胃0.8%CMC;(3)牙周炎小鼠+DMF组:小鼠牙周结扎,每隔2 d龈沟注射LPS并灌胃50 mg/kg DMF悬液,均持续2周。实验结束,取3组小鼠左侧上颌骨进行免疫组织化学染色,检测破骨细胞抑制剂骨保护素(osteoprotegerin,OPG)和核因子κB受体活化因子配体(receptor activator of nuclear factor-κB ligand,RANKL)表达并显微镜观察切片。酶联免疫吸附测定法(enzyme linked immunosorbent assay,ELISA)检测外周血及脾脏白细胞介素-1β(interleukin-1β,IL-1β)表达水平。Micro-CT扫描检测釉牙骨质界至牙槽骨嵴顶(cementoenamel junction to the alveolar bone crest,CEJ-ABC)距离、骨体积分数(bone volume fraction,BV/TV)、骨小梁分离度(trabecular separation,Tb.Sp)和骨小梁厚度(trabecular thickness,Tb.Th),评估牙槽骨丢失情况。苏木精-伊红染色法和抗酒石酸酸性磷酸酶染色,评估组织炎症反应、骨吸收和破骨细胞活性。结果:DMF干预后的牙周炎小鼠CEJ-ABC和Tb.Sp较牙周炎组降低,Tb.Th和BV/TV较牙周炎组增高,外周血及脾脏IL-1β水平较牙周炎组降低,炎症细胞浸润百分比下降,破骨细胞活性及百分比下降。结论:DMF可以减少实验性牙周炎小鼠牙槽骨丢失。
Objective:To investigate the effect of dimethyl fumarate(DMF)on alveolar bone loss in mice with experimental periodontitis induced by ligation.Methods:The groups were as follows:normal mice+solvent group,normal mice were intragastric administrated with 0.8%carboxymethyl cellulose(CMC)every two days;mice with periodontitis+CMC group,periodontal ligation was performed on the mice,lipopolysaccharide(LPS)was injected into the gingival sulcus every two days,and the mice were intragastrically administered with 0.8%CMC;mice with periodontitis+DMF group,periodontal ligation was carried out on the mice,LPS was injected into the gingival sulcus every two days,and the mice were intragastrically administered with 50 mg/kg DMF suspension.The treatments in the latter two groups were lasted for 2 weeks.At the end of the experiment,the left maxilla of three groups of mice was taken for immunohistochemical staining to detect the expression of osteoprotegerin(OPG)and receptor activator of nuclear factor-kB ligand(RANKL),and the sections were observed under a microscope.The expression levels of IL-1β in peripheral blood and spleen were detected by ELISA.Micro-CT was used to scan the distance from the cementoenamel junction to the alveolar bone crest(CEJ-ABC),bone volume fraction(BV/TV),trabecular separation(Tb.Sp),and trabecular thickness(Tb.Th)to evaluate alveolar bone loss.Hematoxylin-eosin staining and tartrate-resistant acid phosphatase staining were used to assess tissue inflammatory response,bone resorption,and osteoclast activity.Results:After DMF intervention,the CEJ-ABC and Tb.Sp of periodontitis mice increased and decreased respectively compared with those in the periodontitis group,while the Tb.Th and BV/TV increased compared with the periodontitis group.The level of IL-1β in peripheral blood and spleen were lower than those in the periodontitis group,the percentage of inflammatory cell infiltration decreased,and the activity and percentage of osteoclasts decreased.Conclusion:DMF can reduce alveolar bone loss in mice with experimental periodontitis.
作者
张雨欣
韦庄
徐雨瑄
蔡明
常志敏
刘岩
ZHANG Yuxin;WEI Zhuang;XU Yuxuan;CAI Ming;CHANG Zhimin;LIU Yan(Xuzhou Medical University,Xuzhou 221004,China;General Dentistry,Xuzhou Stomatological Hospital,Xuzhou 221002,China)
出处
《口腔医学研究》
北大核心
2025年第12期1044-1049,共6页
Journal of Oral Science Research
基金
徐州市卫生健康委科技项目(编号:XWKYHT20240121)。
