摘要
为筛选出适宜徐州地区种植的小麦品种,以16个小麦品种为材料,于6叶期取样,通过主成分分析和系统聚类分析等方法研究不同基因型小麦品种在相同施氮水平下根系结构、干物质量、氮含量及氮积累量差异,并对不同基因型小麦苗期氮效率进行综合评价。结果表明,施氮条件下,不同小麦品种的12个影响氮效率的性状指标存在差异,通过主成分分析将上述12个单项指标转换为4个相互独立的综合指标,累计贡献率达到97.44%。通过对氮效率综合评价值(F值)进行聚类分析,在欧氏距离5处可将供试品种聚成3大类,初步筛选出8个氮高效品种。通过构建小麦苗期氮高效评价的最优回归方程,发现根表面积、根体积、根冠比、根氮含量、植株氮积累量等5项指标对小麦苗期氮高效具有显著影响。此外,筛选出的氮高效小麦品种GS及GOGAT活性均显著高于氮低效品种,TaGS基因表达也呈现出这种趋势,但是TaGOGAT基因表达量未呈现一致规律。
In order to select suitable wheat varieties for cultivation in the Xuzhou Region,16 wheat varieties were selected as samples at the 6-leaf stage.Through methods such as principal component analysis and systematic clustering analysis,the differences in root structure,dry matter weight,nitrogen content,and nitrogen accumulation among different genotypes of wheat varieties under nitrogen application levels were studied.The nitrogen efficiency of different genotypes of wheat seedlings was also comprehensively evaluated.The results showed that under nitrogen application conditions,there were differences in 12 trait indicators affecting nitrogen efficiency among different wheat varieties.Through principal component analysis,the 12 individual indicators were converted into 4 independent comprehensive indicators,with a cumulative contribution rate of 97.44%.Through cluster analysis of the comprehensive evaluation value of nitrogen efficiency(F value),the tested varieties could be clustered into 3 major categories at a euclidean distance of 5.Eight nitrogen-efficient varieties were preliminarily selected.By constructing the optimal equation for nitrogen efficiency in the wheat seedling stage,five indicators such as root surface area,root volume,root-to-shoot ratio,root nitrogen content,and plant nitrogen accumulation had significant importance for nitrogen efficiency in wheat.In addition,the GS and GOGAT activities of the nitrogen-efficient wheat varieties were significantly higher than those of nitrogen-inefficient varieties,and the expression of the TaGS gene also showed this trend,but the expression of the TaGOGAT gene did not show a consistent pattern.
作者
张新钵
郑吴彤
凤舞剑
舒小伟
高峰
张燕霞
强承魁
ZHANG Xinbo;ZHENG Wutong;FENG Wujian;SHU Xiaowei;GAO Feng;ZHANG Yanxia;QIANG Chengkui(College of Agricultural and Forestry Engireering,Xuzhou Vocational College of Bioengineering/Xuzhou Key Laboratory of Modern Agricultural Biotechnology,Xuzhou 221006,China;Xuzhou Jiahe Agricultural Technology Co.,Ltd.,Xuzhou 221200,China)
出处
《黑龙江农业科学》
2025年第12期24-29,36,共7页
Heilongjiang Agricultural Sciences
基金
徐州市科技计划资助(KC23138)
徐州生物工程职业技术学院校级课题(XSZR202202)
徐州佳禾农业科技有限公司科技服务项目(2024320306000417)。
关键词
小麦
氮高效相关性状
主成分分析
聚类分析
wheat
nitrogen-efficient related traits
principal component analysis
cluster analysis
