摘要
1964年我们在非细胞系统用NO_(3)诱导生成硝酸还原酶,首次获得成功。但这项研究由于客观原因中断十几年,从1977年开始我们又继续开展了硝酸还原酶的多方面研究。本文主要介绍硝酸还原酶活力与作物耐肥性的相关性以及在生化育种方面的应用研究结果。
There is a negative correlation between nitrate reductase activity of seedlings and nitrogen response of cultivars among rice,wheat,corn,cotton and other crop plants.The results of studies on nitrate reductase activity in different age of seedlings different nitrate concentrations and inducing time showed that nitrate reductase activity is an inheritable character of cultivar.The variation of nitrate reductase activity among cultivars is regulated mainly by enzyme synthesis and nitrate reductase-inhibitory system,and is not related to the properties of enzyme and the nitrate absorbtion by roots.The nitrate reductase activity in plant is consistent with the level of the nitrate assimilation ability,and is positively related to that of ATP content.These results clearly indicated that cultivars suitable for lower level of nitrogenfertilizer have higher ability of nitrate assimilation and higher ATP content,and are more sensitive to nitrogen fertilizer,while the reverse in them for the cultivars suitable for higher level of nitrogen fertilizer.According to the experiments mentioned above,it is suggested that nitrate reductase activity could be used as a biochemical criterion for nitrogen response of cultivar in crop breeding.The use of nitrate reductase activity in lines selection and new cultivars appraisal has been successful.The new approach to biochemical plant breeding and the use of nitrate reductase in this approach are discussed.
作者
汤玉玮
林振武
陈敬祥
Tang Yuwei;Lin Zhenwu;Chen Jingxiang(Shanghai Institute of plant physiology,Academic Sinica,Shanghai)
出处
《中国农业科学》
1985年第6期39-45,共7页
Scientia Agricultura Sinica
