摘要
CDs作为新型碳基纳米材料凭借其低毒性、优异的光稳定性及发光可调谐性等特性,已成为荧光传感领域的核心材料.传统单发射体系CDs探针依赖荧光强度增强/猝灭现象实现对目标物的检测,此类方法易受探针浓度、微环境波动及仪器参数偏移等因素干扰.为突破这一瓶颈,研究人员引入了具有内部自校准机制的双发射比率荧光探针,以两个或多个荧光信号的强度比值为信号参量,提升了复杂体系中的检测精度与抗干扰能力.本文系统综述了CDs比率荧光探针的最新研究进展,重点剖析了探针类型、设计策略与应用效能.最后,针对当前研究中存在的材料发光原理不明、临床应用转化困难等问题,提出了绿色合成、人工智能辅助设计等未来发展方向.
As a novel carbon-based nanomaterial,CDs have emerged as a core material in fluorescent sensing due to their low toxicity,excellent photostability,and tunable luminescent properties.Traditional single-emission CDs probes rely on fluorescence intensity enhancement or quenching for target detection,which is susceptible to interference from probe concentration,microenvironment fluctuations,and instrumental parameter drifts.To overcome this limitation,ratiometric fluorescent probes with dual-emission systems have been introduced,utilizing an internal self-calibration mechanism based on the intensity ratio of two or more fluorescent signals,thereby enhancing detection accuracy and anti-interference capability in complex systems.This review systematically summarized the latest research progress in CDs-based ratiometric fluorescent probes,with a focus on their design strategies and application performance.Finally,addressing current challenges such as the lack of theoretical guidance in material design and difficulties in practical application transformation,future development directions including green synthesis and artificial intelligence-assisted design are proposed.
作者
王艺霖
李晓琪
赵思思
赵经纬
Yilin Wang;Xiaoqi Li;Sisi Zhao;Jingwei Zhao(Institute of Catalysis for Energy and Environment,College of Chemistry and Chemical Engineering,Shenyang Normal University,Shenyang 110034,China)
出处
《中国科学:化学》
北大核心
2025年第11期3180-3196,共17页
SCIENTIA SINICA Chimica
基金
国家自然科学基金青年科学基金(编号:22109105)
辽宁省属本科高校基本科研业务费(编号:LJ232410166030)
国家级大学生创新创业训练计划项目(编号:202410166025)资助。
关键词
碳点
比率荧光探针
双发射
荧光传感
内部自校准机制
carbon dots
ratiometric fluorescent probes
dual emission
fluorescent sensing
self-calibration
