摘要
茶树春梢萌发期直接影响茶树品种选育与早春茶生产。为提升茶树早生性状的分子辅助育种筛选效率,该研究基于CsAL1区域开发两个KASP分子标记(CsAL1-K1和CsAL1-K4),并在94份材料中进行验证。双标记联合判别在“特早生vs非特早生”场景下准确率达88.3%、精确率达72.7%、漏判率仅7.7%,判别效能高于单标记。两标记分型清晰,基因型间物候期差异显著,适用于特早生/早生材料的高效筛选,可为茶树特早生/早生资源筛选和分子育种提供工具。
The sprouting period of spring shoots in tea plants directly influences cultivar selection and early spring tea production.To enhance the screening for early-sprouting traits in molecular-assisted breeding,two KASP markers(CsAL1-K1 and CsAL1-K4)were developed based on the CsAL1 region and validated using 94 germplasms.In distinguishing"extremely early-sprouting"from"non-extremely early-sprouting"materials,the combined application of both markers achieved an accuracy of 88.3%,a precision of 72.7%,and a miss rate of only 7.7%,demonstrating higher discriminative power than single-marker approaches. Both markers exhibited clear genotyping clustering and significant phenological differences among genotypes, proving suitable for efficient screening of extremely early- and early-sprouting germplasms. This study provides practical tools for the selection of early-sprouting tea resources and molecular breeding programs.
作者
彭律文
雷雨
段继华
丁玎
姚林
饶佳怡
阳虹
谢念祠
陈莹玉
李赛君
刘硕谦
黄飞毅
PENG Lüwen;LEI Yu;DUAN Jihua;DING Ding;YAO Lin;RAO Jiayi;YANG Hong;XIE Nianci;CHEN Yingyu;LI Saijun;LIU Shuoqian;HUANG Feiyi(Hunan Agricultural University,Changsha 410128,China;Tea Research Institute,Hunan Academy of Agricaltural Science/National Center for Tea Improvement in Hunan Branch/National Medium and Small Leaf Tea Plant Germplasm Resource Repository(Changsha),Changsha 410125,China;Yuelushan Laboratory,Changsha 410128,China;Hunan Institute of Microbiology,Changsha 410007,China)
出处
《茶叶通讯》
2025年第4期446-452,共7页
Journal of Tea Communication
基金
湖南省农业科技创新资金(2024CX23,2024CX22)
国家自然科学基金区域联合基金重点项目(U22A20500)
长沙市自然科学基金(Kq2402130)
湖南省重点研发计划项目(2025JK2023)。
关键词
茶树
早生
CsAL1
KASP分子标记
分子标记育种
Camellia sinensis
Early sprouting
CsAL1
KASP molecular marker
Molecular marker breeding
