摘要
建立测定食用植物油中乙基麦芽酚含量的超高效液相色谱-质谱联用仪(UPLC-MS/MS)分析方法。采用Waters HSS T3 C18色谱柱(2.1 mm×50 mm,1.8μm),以0.1%甲酸-水溶液(A)-乙腈溶液(B)为流动相,梯度洗脱(0~1.0 min,5%B;1.0~1.5 min,5%B→95%B;1.5~3.0 min,95%B;3.0~3.5 min,95%B→5%B;3.5~5.0 min,5%B),流速为0.3 mL/min,柱温为40℃,进样量为2μL;质谱采用电喷雾离子源,正离子模式,多反应监测模式(MRM)进行扫描。乙基麦芽酚在0~100 ng/mL范围内与峰面积呈良好的线性关系;平均回收率(n=6)为86.2%~103.0%,相对标准偏差(RSD)为0.63%~5.72%。该方法简便、灵敏、准确、高效,可用于食用植物油中乙基麦芽酚含量的测定。
Ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)analytical method was established for the determination of ethyl maltol content in edible vegetable oils.Waters HSS T3 C18 column(2.1 mm×50 mm,1.8μm)was used as mobile phase with 0.1%formic acid-water solution(A)-acetonitrile solution(B)as mobile phase,gradient elution(0~1.0min,5%B;1.0~1.5min,5%B→95%B;1.5~3.0min,95%B;3.0~3.5 min,95%B→5%B;3.5~5.0 min,5%B),the flow rate was 0.3 mL/min,the column temperature was 40℃,and the injection volume was 2μL.Mass spectrometry scanned the samples using electrospray ion source,positive ion mode,and multiple reaction monitoring mode(MRM).Ethyl maltol had a good linear relationship with the peak area in the range of 0~100 ng/mL;The average recovery rates(n=6)were 86.2%~103.0%,the relative standard deviation(RSD)was 0.63%~5.72%.This method is simple,sensitive,accurate and efficient,and can be used for the determination of ethyl maltol content in edible vegetable oil.
作者
陈娴
黄合琤
曹爱玲
马文思
李彪
金璐
CHEN Xian;HUANG Hecheng;CAO Ailing;MA Wensi;LI Biao;JIN Lu(Kunming Food and Drug Inspection Institute(Kunming Institute of Food and Drug Inspection and Research),Kunming 650032,China;Yunnan Yunce Quality Testing Co.,Ltd.,Kunming 650501,China;Kunming Dongchuan District Market Supervision and Management Comprehensive Service Center,Kunming 654100,China;Kunming Sports School,Kunming 650500,China)
出处
《食品与发酵科技》
2025年第6期125-129,共5页
Food and Fermentation Science & Technology
