摘要
目的采用UPLC-Q-TOF-MS/MS方法,基于多重分析策略研究酸枣仁汤的化学成分。方法采用色谱柱为Waters ACQUITY UPLC BEH C_(18)(2.1 mm×100 mm,1.7μm);流动相为0.1%甲酸水-乙腈,梯度洗脱,流速0.2 mL·min^(-1)。采用信息依赖性采集模式和动态背景扣除的方法,在正、负离子模式下进行数据采集;通过建立化学成分谱库,利用PeakView分析软件对比对照品图谱,根据精确分子量和碎片离子信息,分析质谱裂解规律,并结合相关文献,推断酸枣仁汤中的化学成分;最后应用不同结构的化合物具有不同的Clog P值对同分异构体进行区分。结果采用上述方法共鉴定酸枣仁汤203个化合物,其中42个化合物来自酸枣仁,45个化合物来自川芎,48个化合物来自知母,20个化合物来自茯苓,84个化合物来自甘草,18个化合物来自2味以上的药材。结论该方法全面表征了酸枣仁汤的化学成分谱,为进一步研究其药效物质基础和质量控制提供了参考,同时也可为其他中药化学成分的分析鉴定提供借鉴。
OBJECTIVE To establish an UPLC-Q-TOF-MS/MS method to analyze the chemical constituents of Suanzaoren Decoction(SZRD)based on the multiple analysis strategy.METHODS The chromatographic column was Waters ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7μm)and the mobile phase was 0.1%formic acid-acetonitrile with gradient elution at a flow rate of 0.2 mL·min^(-1).A novel and efficient multiple analysis strategy was developed for identification chemical constituents of SZRD.Firstly,an information-dependent acquisition mode(IDA)method combined with dynamic background subtraction(DBS)was developed to trace all low-level constituents in both positive and negative mode.Secondly,a local chemical composition library was set up for finding compounds,peakView analysis software was used to analysis the chromatogram,accurate molecular mass and fragment ion of standard solutions,the chemical constituents of SZRD were identified by summarizing mass spectrum cracking rules and retrieving literature data.Finally,an important parameter Clog P was used to estimate isomers.RESULTS A total of 203 compounds were identified in SZRD by the above method,42 compounds were derived from Ziziphi Spinosae Semen,45 compounds were derived from Chuanxiong Rhizoma,48 compounds were derived from Anemarrhenae Rhizoma,20 compounds were derived from Poria,84 compounds were derived from Glycyrrhizae Radix et Rhizoma and 18 compounds were from two or more herbs.CONCLUSION This study comprehensively analyzes the chemical composition of SZRD and provides a basis for the further investigation of its pharmacodynamic substances and quality control,and also provides a reference for the analysis and identification of the chemical components of other traditional Chinese medicines.
作者
陈伟燕
李松
郧海丽
高佳丽
杨占坤
靳怡然
赵韶华
刘敏彦
CHEN Weiyan;LI Song;YUN Haili;GAO Jiali;YANG Zhankun;JIN Yiran;ZHAO Shaohua;LIU Minyan(The Second Hospital of Hebei Medical University,Shijiazhuang 050010,China;Hebei Medical University,Shijiazhuang 050017,China;Shijiazhuang Yiling Pharmaceutical Co.,Ltd.,Shijiazhuang 050035,China;Heibei Tibetan Medicine Quality and Technology Innovation Center,Shijiazhuang University,Shijiazhuang 050035,China)
出处
《中国现代应用药学》
北大核心
2025年第19期3382-3396,共15页
Chinese Journal of Modern Applied Pharmacy
基金
河北省自然科学基金资助项目(H2022106048)
石家庄学院博士科研启动基金项目(22BS001)。
