摘要
1985—1987年,在平度县大泽山水库,结合水库的施肥养鱼进行了测验水域的限制性营养元素和最适施肥浓度的生氧量生物测验法的研究。研究结果表明,本方法在灵敏性和再现性方面都是令人满意的。与批次培养法进行的对照测验,证明两种方法的效果基本相同。据此,作者认为,本法用于指导大水域的施肥实践是实用的和可靠的。针对本法一些技术环节所进行的研究,证明在本水库的浮游植物密度(约为200—500万个/L)下,向测验水样接种藻种并非必要,而水样在原水域中的培养时间则以两天为宜。
The oxygen evolution method,a bioassay technique determining the limiting nutrients and the optimum dosages for artificial fertilization,was studied in Dazeshan Reservoir,Ping-du County,Qingdao,during 1985-1987 to obtain an optimun fish farming with artificial fertilization.The general procedure of the method was as follows:water samples collected from 0.5-1.0m depth were filtered through No.20 bolting cloth,and filled into 500 ml colorless glass bottles;then predetermined concentrations or combinations of nutrients and algae culture were added to each bottle.All treatments and the control were triplicate.These bottles were hung horizontally at the depth half way of the Secchi disk measurements;and the dissolved oxygen(DO)of each bottle was determined after a cerlain period of incubation.The nutrients added in those treatments which have larger DO increments than the controls were considered limiting,and the combination or level of the nutrients added in that treatment which has the highest DO increment was considered to be the optimum.This method is satisfactory in both sensitivity and repeatability and as effective as the batch culture method,hence applicable and reliable for guiding artificial fertilization in large water bodies.This method also proves that at the density of 2-5 million phytoplankton per litre as in this reservoir,the inoculation of algae is not indispensable,and the"in situ"incubation time of the samples should be two days.
作者
李德尚
张美昭
赵曼华
许友山
Li Deshang;Zhang Meizhao;Zhao Manhua;Xu Youshan(Ocean University of Qingdao;Water Canservancy Bureau of Pingdu County,Qingdao)
出处
《海洋与湖沼》
1988年第6期539-546,共8页
Oceanologia Et Limnologia Sinica
基金
国家水利电力部的资助项目
