摘要
为建立油莎豆离体再生体系,以油莎豆顶芽为外植体,选用MS为基础培养基,采用正交试验设计研究6-BA、TDZ、ZT组合对油莎豆丛生芽增殖的影响。研究结果表明,3种植物生长调节剂对丛生芽增殖影响的顺序为6-BA>TDZ>ZT,最佳丛生芽诱导培养基为MS+0.5 mg/L 6-BA+0.5 mg/L TDZ+0.7 mg/L ZT。最佳不定根诱导培养基为不添加任何激素的MS培养基,生根率可达100%,平均生根数为5.7条。本研究为高效培育油莎豆优质种苗和优化种质资源提供了重要技术支撑。
To establish an in vitro regeneration system for Cyperus esculentus L.,its apical buds were used as ex⁃plants,and MS was selected as the basic medium.An orthogonal experimental design was adopted to study the ef⁃fects of 6-BA,TDZ,and ZT combinations on the proliferation of cluster buds of Cyperus esculentus L.The results showed that the order of influence of the three plant growth regulators on the proliferation of cluster buds was 6-BA>TDZ>ZT.The optimal medium for cluster bud induction was MS+0.5 mg/L 6-BA+0.5 mg/L TDZ+0.7 mg/L ZT.The optimal medium for adventitious root induction was hormone-free MS medium,with a rooting rate of 100%and an average of 5.7 roots per explant.This study provides important technical support for the efficient culti⁃vation of high-quality seedlings and the optimization of germplasm resources of Cyperus esculentus L.
作者
程艳
张海燕
张健
刘佳遥
魏尊苗
金羽琨
钱雪艳
齐高娃
李红岩
于世超
CHENG Yan;ZHANG Haiyan;ZHANG Jian;LIU Jiayao;WEI Zunmiao;JIN Yukun;QIAN Xueyan;QI Gaowa;LI Hongyan;YU Shichao(Jilin Academy of Agricultural Sciences(Northeast Agricultural Research Center of China),Changchun 130033;Agricultural Technology Promotion Center of Tuquan County,Hinggan League 137500,China)
出处
《东北农业科学》
2025年第4期75-80,共6页
Journal of Northeast Agricultural Sciences
基金
吉林省农业科技创新工程项目(CXGX2023RCG018)
吉林省科技厅重点研发计划项目(20250202023NC)
吉林省现代农业产业技术体系建设示范项目(JLARS-2025-100202)
关键词
油莎豆
组织培养
再生体系
正交试验
生根
Cyperus esculentus L.
Tissue culture
Regeneration system
Orthogonal experiment
Rooting
