摘要
由香蕉炭疽菌(Colletotrichum musae)引发的炭疽病是采后香蕉贮运期间的重要病害,影响果实品质与产业可持续发展。磷脂酶(PL)在植物抗病反应和病原菌代谢中具有双重作用,既是植物防御反应的关键酶,也是病原真菌的毒力因子,在逆境胁迫下使用PL抑制剂有助于维持细胞膜稳定性。本研究通过体外抑菌实验测定3种PL抑制剂——喹吖因二盐酸盐(QU)、新霉素硫酸盐(NE)和正己醇(Hex)对香蕉炭疽菌的抑制效果。结果显示:三者对香蕉炭疽菌菌丝生长和孢子生成均有显著抑制效果,半抑制浓度(IC_(50))分别为1.00、5.00、8.80 mmol/L。PL酶活性抑制试验显示,QU显著抑制香蕉炭疽菌及香蕉果皮PLA活性,NE显著抑制果皮PLC活性,Hex则显著抑制香蕉炭疽菌的PLD活性。此外,适宜浓度的3种PL抑制剂(2.00 mmol/L QU、3.00 mmol/L NE、13.20 mmol/L Hex)均能防控香蕉炭疽病的发展。研究结果表明,QU、NE和Hex通过不同机制干扰病原菌与寄主互作,从而有效控制香蕉炭疽病病害,为香蕉采后病害绿色防控提供理论依据。
Banana anthracnose,caused by Colletotrichum musae,is a major postharvest disease that threatens fruit quality and banana industry sustainability.Phospholipase(PL)plays dual roles in plant disease resistance and pathogen metabolism,acting as a key enzyme in defense responses and as a virulence factor for pathogenic fungi..Previous studies have shown that PL inhibitors can help maintaining membrane stability under stress conditions.In this study,the inhibitory effects of three PL inhibitors,i.e.quinacrine dihydrochloride(QU),neomycin sulfate(NE),and n-hexanol(Hex),on C.musae were evaluated through in vitro antifungal assays.Results showed that all three inhibitors significantly suppressed mycelial growth and spore production of C.musae.The half-maximal inhibitory concentrations(IC_(50))for mycelial growth were 1.00 mmol/L(QU),5.00 mmol/L(NE),and 8.80 mmol/L(Hex).Enzymatic activity assays revealed that QU significantly inhibited both PLA activity in C.musae and banana peel tissues,NE primarily suppressed PLC activity in banana peel,whereas Hex markedly reduced PLD activity in C.musae.Moreover,the appropriate concentrations of the inhibitors(2.00 mmol/L QU,3.00 mmol/L NE,and 13.20 mmol/L Hex)effectively controlled the development of banana anthracnose.The findings suggested that QU.NE,and Hex suppressed banana anthracnose through distinct mechanisms involving interference with host-pathogen interactions.This study would provide a theoretical basis for the green control of postharvest banana diseases via the targeted regulation of phospholipase activity.
作者
董昊
黄春霞
程秋芋
谢丽红
易萍
王淑培
韦琦
黄方
隆宇涵
李丽
孙健
DONG Hao;HUANG Chunxia;CHENG Qiuyu;XIE Lihong;YI Ping;WANG Shupei;WEI Qi;HUANG Fang;LONG Yuhan;LI Li;SUN Jian(Guangxi University,Nanning,Guangxi 530004,China;Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China;Guangxi Key Laboratory of Fruit and Vegetable Storage-processing Technology,Nanning,Guangxi 530007,China)
出处
《热带作物学报》
北大核心
2025年第11期2713-2721,共9页
Chinese Journal of Tropical Crops
基金
广西科技基地和人才专项(桂科AD24010045)
国家自然科学基金项目(No.32272784,No.32160732)。
关键词
香蕉
炭疽病菌
磷脂酶抑制剂
磷脂酶活性
banana
Colletotrichum musae
phospholipase inhibitors
phospholipase activity
