摘要
盐胁迫是制约植物生长发育的主要非生物胁迫之一.在设施农业领域,过量施用化肥引发的土壤次生盐渍化问题日益严峻.PIFs在调控种子萌发、生物节律、激素信号及逆境响应等方面具有重要作用.辣椒中PIF家族成员的生物学功能尚未全部被鉴定.本研究发现,CaPIF1基因在各个组织均有表达,而在叶中的相对表达水平最高;CaPIF1基因的表达受盐诱导,在8 h相对表达量达到最大值;具有转录活性,并定位于细胞核;通过VIGS技术,获得CaPIF1基因沉默的辣椒植株(pTRV-CaPIF1);发现300 mmol/L NaCl处理后,pTRV-CaPIF1植株与空载组(CK)相比,其萎蔫程度更为严重;丙二醛(MDA)含量显著高于对照组,脯氨酸(Pro)含量在盐胁迫后显著下降,ABA含量下降,Na+/K+比值升高;pTRV2:CaPIF1植株中H2O2和O2-的积累更为显著,以上数据说明,CaPIF1基因参与辣椒盐胁迫响应,沉默该基因导致植株耐盐性显著降低.RNA-seq数据分析发现:共有189个差异基因出现在盐处理0 h和8 h的4个不同组别中,其中包括参与非生物胁迫响应的重要基因和ABA信号途径相关基因包括CaRAS1,CaMPK3,CaNFXL2,CaNCED1,CaABAH2,CaRSM1及CaPYL4.GO功能富集分析显示,差异基因主要集中在信号转导、激酶活性、翻译、胚胎后发育、细胞质蛋白修饰过程、DNA结合及RNA结合等条目.KEGG分析结果显示:最显著富集的5条通路分别是MAPK信号通路、植物激素信号转导、蛋白加工途径及淀粉和蔗糖代谢.综上所述,本研究推测CaPIF1作为转录因子可能通过ABA信号转导通路调控盐胁迫响应.
Salt stress is one of the main abiotic stresses that restrict plant growth and development.In the field of agricultural production,the problem of secondary salinization of soil caused by excessive fertilizer application has become increasingly severe.The phytochrome-interacting factors(PIFs),which belong to the basic helix-loop-helix(bHLH)transcription factor family,play a crucial role in regulating various physiological processes in plants,including seed germination,circadian rhythms,hormone signaling,and sugar metabolism.PIFs can also regulate plant responses to abiotic stresses through pathways involving abscisic acid(ABA),gibberellic acid(GA),and ethylene.However,the members of the PIF family have not been fully identified on paper.In this study,CaPIF1 was found to contain a conserved bHLH domain,possess transcriptional activity,and localize in the nucleus.The expression of CaPIF1 was detected in various tissues,with the highest relative expression observed in leaves.The expression of CaPIF1 was induced by salt stress,reaching its maximum at 8 hours after salt stress treatment.Through the VIGS technique,CaPIF1-silenced pepper plants(pTRV-CaPIF1)were obtained.After treatment with 300 mmol/L NaCl,the pTRV-CaPIF1 plants showed more severe wilting compared to the control group(CK).Under high salinity stress,the accumulation of H 2O 2 and O 2−in the pTRV2:CaPIF1 plants was significantly higher,and the MDA content and the Na+/K+ratio were significantly increased,while the proline(Pro)content and ABA content were decreased significantly.These results indicate that the salt tolerance of the pTRV-CaPIF1 plants was reduced,and the CaPIF1 gene participates in the salt stress response in pepper.RNA-seq data analysis revealed that a total of 189 differentially expressed genes were identified across the 4 distinct groups before/after salt treatment,including many important genes involved in abiotic stress response genes and ABA signaling pathway-related genes,such as CaRAS1,CaMPK3,CaNFXL1,CaNCED1,CaABAH2,and CaPYL4.GO functional enrichment analysis showed that the differentially expressed genes were mainly enriched in signal transduction,kinase activity,translation,postembryonic development,cytoplasmic protein modification,DNA binding,and RNA binding.KEGG analysis revealed that the five most significantly enriched pathways were the MAPK signaling pathway,plant hormone signaling transduction,protein processing,and starch and sucrose metabolism.Therefore,we hypothesize that CaPIF1,as a transcription factor,participates in the salt stress response through the ABA signaling transduction pathway.
作者
李慧
王悦
文钰芳
纪超
石国优
杨有新
刘建萍
LI Hui;WANG Yue;WEN YuFang;JI Chao;SHI GuoYou;YANG YouXin;LIU JianPing(Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources,College of Bioscience and Bioengineering,Jiangxi Agricultural University,Nanchang 330045,China;Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits&Vegetables,College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China)
出处
《中国科学:生命科学》
北大核心
2025年第11期2383-2404,共22页
Scientia Sinica(Vitae)
基金
江西省自然科学基金(批准号:S2024ZRZDL0428,20232BAB205041,20212ACB215006)
国家自然科学基金(批准号:32060047)
江西省教育厅项目(批准号:GJJ2200441)资助。
