摘要
以‘苏翠1号’梨花芽为试材,比较正常花芽和松散花芽的赤霉素含量,转录组比较分析赤霉素合成途径相关基因表达情况,研究施加赤霉素抑制剂多效唑控制花芽松散的效果,探讨花芽松散与赤霉素之间的关系。结果表明,松散花芽中赤霉素含量显著高于正常花芽,其中GA_(1)、GA_(3)、GA_(4)和GA_(7)含量是正常花芽的1.25~2.87倍。转录组分析显示松散花芽与正常花芽有3290个差异表达基因,松散花芽中上调表达的2046个,下调表达的1244个。qRT-PCR结果表明,松散花芽中GA2ox1、GA2ox2、GA3ox、GA20ox和GID1基因上调表达,GAI、GASA3/4/6/14、GID2和KAO下调表达,并且与转录组测序结果一致。施加多效唑后一年生枝条腋花芽中赤霉素含量减少,花芽松散比例下降,赤霉素含量与花芽松散比例呈显著正相关(r=0.94)。喷施多效唑可抑制‘苏翠1号’梨腋花芽中GA2ox1/2、GA3ox、GA20ox、GASA3/4/6/14、GID1和KAO的转录水平,促进GAI和GID2表达,从而调控赤霉素的合成代谢。研究结果表明生长季赤霉素的异常升高会导致梨花芽松散,喷施多效唑通过调控合成途径相关基因表达,抑制赤霉素合成,减轻梨花芽的松散程度。
To explore the relationship between flower buds wizening and gibberellin,the flower bud from Pyrus pyrifolia cultivar‘Sucui 1’was selected as the experimental material in this study.The gibberellin contents of‘Sucui 1’pear were compared between normal flower buds and wizened flower buds,and the expression of genes related to the gibberellin synthesis pathway once flower buds wizening were analyzed.Then the effect of applying gibberellin inhibitor paclobutrazol to control flower buds wizening was studied in the growing season.The results showed that there was significant difference in gibberellin contents between normal flower buds and wizened flower buds.The contents of GA_(1),GA_(3),GA_(4)and GA_(7)in wizened flower buds were 1.25–2.87 times of those in normal flower buds.Transcriptome analysis showed that,comparing with normal flower buds,wizened flower buds had 3290 differentially expressed genes in which 2046 were up-regulated and 1244 were down-regulated.qRT-PCR confirmed the up-regulated expression of GA2ox1(gibberellin 2-oxidas 1),GA2ox2(gibberellin 2-oxidas 2),GA_(3)ox(gibberellin 3-oxidas),GA20ox(gibberellin 20-oxidas)and GID1(gibberellin insensitive Dwarf1)genes in wizened flower buds.Meanwhile,GAI(gibberrellic acid insensitive),GASA_(3)/4/6/14(Arabidopsis gibberrellic acid stimulating protein 3,4,6 and 14),GID2(gibberellin Insensitive Dwarf2)and KAO(ent-kaurenoic acid monooxygenase)were down-regulated in wizened flower buds.And their expression information was consistent with the results of transcriptome sequencing.After the application of polybulobuzole,the gibberellin contents in axillary flower buds of annual branches reduced and the proportion of wizened flower buds in‘Sucui 1’decreased in mid-September.The content of gibberellin was significantly positively correlated with the proportion of wizened flower buds(r=0.94).Furthermore,the transcription levels of GA2ox1/2,GA_(3)ox,GA20ox,GAI,GASA_(3)/4/6/14,GID1/2 and KAO in‘Sucui 1’flower bud were affected by applying polybulobuzole,which took part in the synthesis and metabolism of gibberellin.Spraying paclobutrazol can inhibit the transcription levels of GA2ox1/2,GA_(3)ox,GA20ox,GASA_(3)/4/6/14,GID1,and KAO in the axillary flower buds of‘Sucui 1’pear,but promote the expression of GAI and GID2,furthermore regulate the synthesis and metabolism of gibberellin.The research results indicate that an abnormal increase in gibberellin during the growing season can lead to wizened flower buds in pear.Spraying paclobutrazol would regulate the expression of genes related to the gibberellin synthesis pathway,inhibit it synthesis,and relief the wizened degree of pear flower buds.
作者
李慧
阚家亮
李晓刚
王中华
蔺经
杨青松
LI Hui;KAN Jialiang;LI Xiaogang;WANG Zhonghua;LIN Jing;YANG Qingsong(Institute of Pomology,Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210014,China;State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,China)
出处
《园艺学报》
北大核心
2025年第10期2713-2726,共14页
Acta Horticulturae Sinica
基金
江苏省农业科技自主创新资金项目[CX(22)3052]
江苏省种业振兴揭榜挂帅项目[JBGS[2021]084]
江苏现代农业产业技术体系建设项目[JATS[2023]396]
国家梨产业技术体系徐州综合试验站(CARS-28-33)。
关键词
梨
花芽松散
转录组
赤霉素
基因表达
pear
flower bud wizening
transcriptome
gibberellin
gene expression
