摘要
为优化炒槐米总黄酮的双水相提取工艺,并评估其抗氧化活性。采用异丙醇-磷酸氢二钾为双水相体系,以总黄酮得率为评价指标,以异丙醇质量分数、磷酸氢二钾质量分数、液料比、提取温度及提取时间等因素为自变量,在单因素试验基础上,利用响应面法优化提取工艺,并以VC为阳性对照,通过测定其对1,1-二苯基-2-三硝基苯肼(DPPH)自由基和羟基自由基的清除能力评估炒槐米总黄酮的抗氧化活性。结果表明,炒槐米总黄酮的最佳提取条件为:异丙醇质量分数37%,磷酸氢二钾质量分数12%,液料比86∶1(mL/g),提取温度40℃,提取时间1 h。在此条件下,总黄酮的预测得率为17.81%,试验验证得率为17.70%,证实了模型的准确性。此外,炒槐米总黄酮展现出一定的抗氧化活性,炒槐米总黄酮质量浓度为0.1150 mg/mL时对DPPH自由基和羟基自由基的清除率分别为72.50%和37.88%。综上,异丙醇-磷酸氢二钾双水相体系提取炒槐米总黄酮工艺稳定可行,可为槐米资源的开发利用提供理论基础。
To optimize the aqueous two-phase extraction process of total flavonoids from stir-fried Flos Sophorae Immaturus,response surface methodology was implemented based on single factor experiments by using isopropanol-dipotassium hydrogen phosphate as the aqueous two-phase system,the yield of total flavonoids as the index and factors such as mass fraction of isopropanol,mass fraction of dipotassium hydrogen phosphate,liquid-to-material ratio,extraction temperature,and extraction time as independent variables.With ascorbic acid used as the positive control,the antioxidant properties of total flavonoids from stir-fried Flos Sophorae Immaturus were evaluated by measuring its capacity to scav-enge 1,1-diphenyl-2-picrylhydrazyl(DPPH)and hydroxyl free radicals.Results showed that the optimal extraction conditions for total flavonoids from stir-fried Flos Sophorae Immaturus were as follows:mass fraction of isopropanol 37%,mass fraction of dipotassium dihydrogen phosphate 12%,liquid-to-material ratio 86:1(mL/g),extraction tem-perature 40℃,and extraction time 1 h.Under these conditions,the predicted yield was 17.81%,while the experimen-tally verified yield was 17.70%,and the similarity between the two confirms the accuracy of the model.In addition,when the mass fraction of total flavonoids from stir-fried Flos Sophorae Immaturus was 0.1150 mg/mL,the total flavonoids from stir-fried Flos Sophorae Immaturus exhibited certain antioxidant properties,with scavenging rates of 72.50%and 37.88%on DPPH and hydroxyl free radicals,respectively.The extraction process of total flavonoids from stir-fried Flos Sophorae Immaturus using the aqueous two-phase system of isopropanol-dipotassium hydrogen phosphate is stable and feasible,which can provide a theoretical basis for the development and utilization of Flos Sophorae Immaturus resources.
作者
邓博文
潘星旭
王昊
邱星豪
王文彬
俞杰
饶桂维
Zhylko Viachaslau
DENG Bowen;PAN Xingxu;WANG Hao;QIU Xinghao;WANG Wenbin;YU Jie;RAO Guiwei;ZHYLKO Viachaslau(College of Biology and Environmental Engineering,Zhejiang Shuren University,Hangzhou 310015,China;Interdisciplinary Research Academy,Zhejiang Shuren University,Hangzhou 310015,China;Faculty of Chemistry,Belarusian State University,Minsk 220072,Republic of Belarus)
出处
《保鲜与加工》
北大核心
2025年第11期91-99,共9页
Storage and Process
基金
科技部“一带一路”创新人才交流外国专家项目(DL2023016004L)。
关键词
炒槐米
总黄酮
双水相体系
提取工艺
抗氧化活性
stir-fried Flos Sophorae Immaturus
total flavonoids
aqueous two-phase system
extraction process
