摘要
目的:探究凝结魏茨曼氏菌CGMCC 9951对酒精诱导的肝脏损伤缓解效果和对肠道菌群的调节作用。方法:建立酒精性肝损伤小鼠模型,并用凝结魏茨曼氏菌CGMCC 9951低、中、高剂量干预4周后,测定行为学指标、生理生化指标、抗氧化指标并分析肠道菌群变化。结果:凝结魏茨曼氏菌CGMCC 9951可显著延长小鼠醉酒潜伏期,缓解酒精造成的脏器指数异常,降低血清ALT、AST水平,提高肝脏抗氧化能力(P<0.05);菌群多样性分析显示各组菌群结构差异明显,在门水平上,凝结魏茨曼氏菌CGMCC 9951干预使拟杆菌门丰度升高,疣微菌门丰度降低;在属水平上,乳杆菌、优杆菌等有益菌属丰度升高,肠杆菌等条件致病菌属丰度降低;功能预测显示,凝结魏茨曼氏菌CGMCC 9951可能通过色氨酸代谢通路、丁酸盐代谢通路等发挥作用。结论:凝结魏茨曼氏菌CGMCC 9951可能通过提高抗氧化能力,升高肠道内有益菌丰度,降低致病菌丰度,来维持肠道菌群的稳定,从而改善酒精性肝损伤。
Objective:The mitigative effect of Weizmannia coagulans CGMCC 9951 on alcohol-induced liver injury and its regulatory effect on intestinal microbiota were investigated.Methods:An alcohol-induced liver injury mouse model was established,and Weizmannia coagulans CGMCC 9951 was intervened at low,medium,and high doses for 4 weeks.Behavioral,physiological,biochemical,and antioxidant indicators were measured,and the changes in the intestinal microbiota were analyzed.Results:Weizmannia coagulans CGMCC 9951 significantly prolonged the drunk incubation period,alleviated alcohol-induced organ index abnormalities,lowered serum ALT and AST levels,and improved the antioxidant capacity of the liver in mice(P<0.05).The analysis of microbial diversity showed significant differences in the microbial structure among groups.At the phylum level,intervention of Weizmannia coagulans CGMCC 9951 increased the abundance of Firmicutes and decreased the abundance of Verrucomicrobiota.At the genus level,the abundance of beneficial genera Lactobacillus and Eubacteria increased,while the abundance of pathogenic genus Enterobacter decreased.Functional prediction showed that Weizmannia coagulans CGMCC 9951 might play a role through tryptophan metabolism and butanoate metabolism pathways.Conclusion:Weizmannia coagulans CGMCC 9951 may improve alcoholic liver injury by enhancing antioxidant capacity,increasing the abundance of beneficial bacteria in the gut,reducing the abundance of pathogenic bacteria,and maintaining the stability of the gut microbiota.
作者
李成
吴影
郭明涵
段梦瑶
张杰
古绍彬
LI Cheng;WU Ying;GUO Minghan;DUAN Mengyao;ZHANG Jie;GU Shaobin(College of Food and Bioengineering,Henan University of Science and Technology,Luoyang 471023,China;Henan Engineering Research Center of Food Microbiology,Luoyang 471023,China;National Demonstration Center for Experimental Food Processing and Safety Education,Luoyang 471023,China)
出处
《食品工业科技》
北大核心
2025年第23期212-220,共9页
Science and Technology of Food Industry
基金
国家自然基金青年基金项目(32302069)
河南省青年托举人才项目(20230035)。
关键词
凝结魏茨曼氏菌
酒精性肝损伤
肠道菌群
益生菌
Weizmannia coagulans
alcoholic liver disease
intestinal flora
probiotics
