摘要
该研究通过氧化偶氮甲烷(azoxymethane,AOM)和硫酸葡聚糖钠(dextran sodium sulfate,DSS)诱导小鼠炎症相关性结直肠癌模型,探究分离自新疆传统发酵酸乳中的植物乳植杆菌KSFY01(Lactiplantibacillus plantarum KSFY01,LP-KSFY01)对实验小鼠的作用效果及机制。实验设置正常组、模型组、阿司匹林组以及LP-KSFY01低、高浓度(1×10^(8)、1×10^(9)CFU/kg)组,除正常组外,各组小鼠均用AOM/DDS处理,阿司匹林组每日灌胃67 mg/kg阿司匹林,菌组每日灌胃LP-KSFY01菌悬液。实验测定小鼠体重、脏器质量、结直肠长度,统计肠组织肿瘤数,对直肠进行组织病理学分析;利用酶联免疫吸附法检测小鼠血清和结肠中炎症细胞因子含量;实时荧光定量PCR测定结肠中NF-κB和细胞凋亡通路相关因子mRNA表达。实验结果显示LP-KSFY01干预能显著缓解体重减轻和脏器指数异常,减少肠道肿瘤发生率,改善肠道组织病理学损伤,降低血清和结肠中IL-6、IL-1β、TNF-α、核因子κB(nuclear factorκB,NF-κB)和诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)等炎症细胞因子含量,下调结肠中IκBβ、p65、p50、p52、Bcl-2和Bcl-x L基因的表达水平,提高Bid和caspase-8基因的表达水平(P<0.05),且高浓度的LP-KSFY01效果优于低浓度。综上,LP-KSFY01可通过调节NF-κB和细胞凋亡途径,发挥缓解肠道炎症进程和干预肠道肿瘤发展的作用,表明LP-KSFY01对小鼠炎症相关性结直肠癌具有潜在的调节作用,有进一步研究的价值。
A mouse model of inflammation-related colorectal cancer was established through the induction of azoxymethane(AOM)and dextran sodium sulfate(DSS)to investigate the effects and mechanisms of Lactiplantibacillus plantarum KSFY01(LP-KSFY01),isolated from traditional fermented yogurt in Xinjiang.The experimental design included a normal group,a model group,an aspirin group,as well as groups treated with low(1×10^(8)CFU/kg)and high(1×10^(9)CFU/kg)concentrations of LP-KSFY01.Except for the normal group,all mice underwent treatment with AOM/DSS.The aspirin group received daily oral doses of 67 mg/kg of aspirin,while the LP-KSFY01 groups were administered daily oral suspensions of LP-KSFY01.Measurements were taken of body weight,organ weight,colon length,and the number of intestinal tumors,along with histopathological analyses of the rectum.Enzyme-linked immunosorbent assay was utilized to assess the levels of inflammatory cytokines in serum and colon tissues,and real-time fluorescence quantitative PCR was employed to measure the mRNA expression of NF-κB and apoptosis-related factors in the colon.Results indicated that LP-KSFY01 intervention significantly mitigated weight loss and normalized organ indices,reduced the incidence of intestinal tumors,and improved histopathological damage in the rectum.Furthermore,a decrease in the levels of inflammatory cytokines such as IL-6,IL-1β,TNF-α,and iNOS was observed in both serum and colon tissues(P<0.05).The mRNA expression of IκBβ,p65,p50,p52,Bcl-2,and Bcl-x L was downregulated,while the expression of Bid and caspase-8 was upregulated(P<0.05).Notably,a greater effect was noted with the high concentration of LP-KSFY01 compared to the low concentration.In conclusion,LP-KSFY01 may play a role in alleviating intestinal inflammation progression,intervening in colorectal tumor development through modulating the NF-κB and apoptosis pathways,indicating a potential regulatory effect on inflammation-related colorectal cancer in mice and suggesting further research value.
作者
余婷
赵欣
胡甜甜
YU Ting;ZHAO Xin;HU Tiantian(Collaborative Innovation Center for Child Nutrition and Health Development,Chongqing Engineering Research Center of Functional Food,Chongqing Engineering Laboratory for Research and Development of Functional Food,Chongqing University of Education,Chongqing 400067,China)
出处
《食品与发酵工业》
北大核心
2025年第17期108-117,共10页
Food and Fermentation Industries
基金
重庆市教育委员会科学技术研究计划青年项目资助项目(KJQN202201601)
重庆市自然科学基金面上项目(cstc2021jcyj-msxmX0424)。
关键词
植物乳植杆菌
氧化偶氮甲烷/硫酸葡聚糖钠
结直肠癌
NF-ΚB
细胞凋亡
Lactiplantibacillus plantarum
azoxymethane(AOM)/dextran sodium sulfate(DSS)
inflammation-associated colorectal cancer
NF-κB
apoptosis
