摘要
目的:建立绞股蓝流式细胞术的倍性和基因组大小检测方法。方法:通过比较不同细胞核提取液、过滤方式、取样部位及电压条件,筛选出绞股蓝倍性检测最佳方案,并以二倍体为参照,完成对42份种质资源的倍性与基因组大小检测。结果:以绞股蓝嫩叶为材料,用LB01细胞核提取液进行细胞核提取,经70μm细胞筛过滤后在422V电压条件下进行倍性检测的效果最佳。42份绞股蓝种质共检测出二倍体18份、四倍体20份、六倍体4份。不同种质绞股蓝的基因组大小存在较大差异,基因组大小在513.25~1294.86Mb之间。结论:基于流式细胞术建立了绞股蓝倍性鉴定和基因组大小评估方法,为绞股蓝种质资源鉴定及种质创新利用提供了技术支持。
Objective:To establish a flow cytometry method for determining the ploidy and genome size detection of Gynostemma pentaphyllum.Methods:By comparing different nuclear extracts,filtration methods,sampling sites and voltage conditions,the best scheme for ploidy detection of G.pentaphyllum was screened out,and the ploidy levels and genome size of 42 germplasm resources were determined using diploid as a reference.Results:The results demonstrated that the optimal effect for ploidy detection was obtained when using young leaves of G.pentaphyllum as the material.The nuclei were extracted with LB01 nuclear extraction buffer,followed by filtration through a 70μm cell strainer,and then detection was carried out under a voltage of 422 V.A total of 18 diploid,20 tetraploid,and 4 hexaploid samples were identified among the 42 G.pentaphyllum germplasm resources.The genome sizes varied significantly among different germplasm resources,ranging from 513.25 Mb to 1294.86 Mb.Conclusion:A method for ploidy identification and genome size assessment of G.pentaphyllum has been established based on flow cytometry,providing technical support for the identification and innovative utilization of G.pentaphyllum germplasm resources.
作者
池玉珍
余丽莹
周芸伊
姚李祥
何龙飞
潘春柳
王爱勤
CHI Yuzhen;YU Liying;ZHOU Yunyi;YAO Lixiang;HE Longfei;PAN Chunliu;WANG Aiqin(College of Agriculture,Guangxi University,Nanning 530004,China;Guangxi TCM Resources General Survey and Data Collection Key Lab.,Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China)
出处
《中国野生植物资源》
2025年第10期29-35,共7页
Chinese Wild Plant Resources
基金
国家自然科学基金项目(32260071)
广西中医药适宜技术开发与推广项目(GZSY-23-06)
国家现代农业产业技术体系(CARS-21)。
关键词
绞股蓝
流式细胞术
倍性鉴定
基因组大小
Gynostemma pentaphyllum
Flow cytometry
Ploidy identification
Genome size
