摘要
目的探讨光生物调节(PBM)是否通过影响三磷酸腺苷(ATP)含量及腺苷受体含量,进而改善慢性应激导致的认知功能障碍。方法24只C57BL/6J小鼠随机分为对照组、应激组和治疗组,采用慢性不可预见性温和刺激方法对小鼠进行造模,于造模第6周时对治疗组小鼠给予PBM干预,干预持续1周后进行行为学检测,观察小鼠行为变化;蛋白质免疫印迹实验(WB)检测3组小鼠海马及前额叶中A1、A2B、A3型3种腺苷受体的表达变化。12只C57BL/6J小鼠随机分为对照组和干预组,对干预组小鼠进行1周PBM干预,干预结束后进行行为学检测;WB检测对照组和干预组小鼠海马与前额叶内的A1、A2B、A3型3种腺苷受体变化;免疫荧光法检测对照组和干预组小鼠海马c-Fos的表达情况;碧云天ATP检测试剂盒检测对照组和干预组小鼠海马与前额叶组织内ATP含量变化。进一步进行细胞实验验证PBM对细胞内ATP含量变化的影响。结果与对照组相比,应激组小鼠的移动距离未发生显著变化,但穿台次数显著减少;治疗组穿台次数与对照组无显著差异,与应激组相比穿台次数显著增加。应激组小鼠的海马和前额叶中的腺苷受体水平显著降低,治疗组小鼠海马和前额叶中腺苷受体水平升高。干预组小鼠穿台次数显著高于对照组,海马和前额叶中腺苷受体水平、ATP含量升高,海马c-Fos表达增加。结论PBM可能通过调节ATP水平及腺苷受体含量,进而调控海马脑区神经元响应程度,最终改善慢性应激诱导的认知功能障碍。
Objective To find out whether photobiomodulation(PBM)can mitigate cognitive dysfunction caused by chronic stress by affecting levels of adenosine triphosphate(ATP)and adenosine receptors.Methods Twenty-four C57BL/6J mice were randomly divided into a control group,a stress group,and a treatment group.Chronic unpredictable mild stress was used to establish a mouse model of stress.Six weeks into modeling,the treatment group was subjected to one week of PBM interventions.Behavioral tests were conducted to observe behavioral changes in the mice.Western blotting(WB)was used to detect the expressions of A1,A2B,and A3 adenosine receptors in the hippocampus and prefrontal cortex of mice in the three groups.Twelve C57BL/6J mice were randomly divided into a control group and an intervention group.The intervention group received a week of PBM interventions and underwent behavioral testing.WB was used to detect the expression changes of A1,A2B,and A3 adenosine receptors in the hippocampus and prefrontal cortex in both groups.Immunofluorescence assay was adopted to detect the expression of c-Fos in the hippocampus of mice in the two groups.The ATP assay kit made by Beyotime Biotechnology Co.,Ltd.was used to measure changes in ATP contents in the hippocampus and prefrontal cortex tissues of mice.Cell experiments were conducted to verify the effect of PBM on intracellular ATP contents.Results Mice in the stress group covered a similar distance to the control group,but finished far fewer platform crossings.There was no significant difference between the treatment group and the control group in the number of times of platform crossings,but compared favorably with the stress group where the levels of adenosine receptors in the hippocampus and prefrontal cortex were lower,but were increased by PBM.After PBM interventions in normal mice,platform crossings were increased significantly compared to the control group.PBM also raised adenosine receptor levels and ATP contents in the hippocampus and prefrontal cortex,and increased hippocampal c-Fos expressions.In vitro,PBM elevated intracellular ATP levels.Conclusion PBM may improve chronic stress-induced cognitive dysfunction by regulating ATP levels and adenosine receptor expressions,thereby modulating neuronal responsiveness in the hippocampus.
作者
董华丰
刘冰
陈小冰
刘薇薇
谢方
赵云
孙兆炜
王雪
钱令嘉
DONG Huafeng;LIU Bing;CHEN Xiaobing;LIU Weiwei;XIE Fang;ZHAO Yun;SUN Zhaowei;WANG Xue;QIAN Lingjia(Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China)
出处
《军事医学》
2025年第9期647-654,共8页
Military Medical Sciences
