摘要
霍山石斛(Dendrobium huoshanense)是兰科国家一级保护野生濒危植物,也是一种药食兼用的中药资源植物。虽然设施和林下栽培已经成为霍山石斛的主要栽培模式,但两种栽培模式下霍山石斛地上部生物量与基质/土壤中微生物群落间的关联特征尚不清楚,限制了关于霍山石斛地上-地下过程与机制的理解。鉴于此,该研究在安徽大别山霍山石斛种植基地开展了野外实验与随机采样,旨在探究霍山石斛设施和林下栽培模式中养分对植物-微生物关联的调控。结果发现:设施和林下栽培模式下,基质与土壤中的微生物生物量、多样性与群落组成均存在显著的差异,具体表现在基质中微生物生物量碳、氮含量显著高于林下土壤,且具有更丰富的细菌多样性与丰度更高的外生菌根真菌。林下种植霍山石斛显著改变了土壤中的微生物群落,而在设施栽培模式下种植霍山石斛却没有改变基质中的微生物群落。基质中霍山石斛植株生物量显著高于林下。此外,结构方程模型结果表明,基质/土壤中的养分含量和微生物群落组成与霍山石斛植株生物量之间存在密切的调控关系,且不同栽培模式下这种关系有所不同。具体地,在设施栽培的富养条件下,外生菌根真菌直接促进了霍山石斛植株的地上部生物量;而在林下栽培的贫养条件下,病原菌的增殖可能会抑制霍山石斛植株的地上部生长。该研究理清了设施和林下栽培霍山石斛模式中植株生物量与微生物群落之间的关联性,为开发促进植株生长的功能菌剂、保护霍山石斛这一珍稀濒危植物资源提供了科学依据。
Aims Dendrobium huoshanense(Orchidaceae)is a nationally protected and endangered medicinal plant with dual-purpose(medicinal and edible)value in China.While facility and understory cultivation dominate its artificial propagation,the linkages between aboveground biomass and substrate/soil microbial communities remain unclear,hindering the understanding of aboveground-belowground association.Methods Hence,we conducted field experiments with randomized sampling at a D.huoshanense cultivation base in the Dabie Mountains,Anhui,to investigate nutrient-regulated plant-microbe association under facility vs understory modes.Important findings Facility substrates and understory soil showed significant differences in microbial biomass,diversity,and community composition.Specifically,facility substrates had higher microbial biomass carbon(MBC)and nitrogen(MBN)contents than understory soil,along with enriched bacterial diversity and ectomycorrhizal fungi(ECM).While understory cultivation significantly altered soil microbial composition,facility substrates maintained stable microbial communities.The biomass of D.huoshanense in the facility substrate was significantly higher than that in the understory soil.Further,structural equation modeling results revealed that nutrient content in substrate/soil and the composition of microbial communities exhibit significant regulatory associations with the biomass of D.huoshanense plants,with distinct patterns emerging under varying cultivation regimes.Specifically,ECM fungi under nutrient-rich facility conditions directly promoted aboveground biomass,whereas pathogen proliferation in nutrient-limited understory soils might suppress aboveground growth.These findings clarify the associations between aboveground biomass and microbial communities in the facility and understory cultivation modes of D.huoshanense,providing actionable insights for developing microbial inoculants to enhance cultivation efficiency and conserve this endangered species.
作者
朱瑞德
杨俊薇
刘宵含
陈冰瑞
池秀莲
田地
杨光
程蒙
戴亚峰
王诗文
ZHU Rui-De;YANG Jun-Wei;LIU Xiao-Han;CHEN Bing-Rui;CHI Xiu-Lian;TIAN Di;YANG Guang;CHENG Meng;DAI Ya-Feng;WANG Shi-Wen(State Key Laboratory of Efficient Production of Forest Resources,Beijing Forestry University,Beijing 100083,China;State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Jiuxianzun Huoshan Dendrobium Co.,Ltd.,Lu’an,Anhui 237000,China)
出处
《植物生态学报》
北大核心
2025年第9期1434-1447,共14页
Chinese Journal of Plant Ecology
基金
国家自然科学基金(82173930)
中国中医科学院科技创新工程项目(CI2021A03908和CI2023E002)。
关键词
霍山石斛
林下栽培
设施栽培
土壤微生物
生物量
养分
地上-地下
Dendrobium huoshanense
understory cultivation
facility cultivation
soil microbe
biomass
nutrient
above-and below-ground
