摘要
茄科作物作为全球重要作物,涵盖马铃薯、番茄、辣椒、烟草等关键粮食、蔬菜及经济作物,其产量和品质直接影响农业发展与人类健康。然而,茄科作物在生产过程中常面临生物胁迫和非生物胁迫等问题,需通过基因编辑、转基因等遗传改良技术提升其抗逆性和产量。发根农杆菌介导的转化因其宿主范围广、操作简便、无需组织培养、稳定遗传且适用于根系相关基因功能解析,已成为茄科作物分子育种和基因功能研究的重要工具。综述了发根农杆菌Ri质粒的分类与结构特征及其在茄科作物中的应用,重点探讨了发根农杆菌在基因功能验证与抗性育种中的创新实践,并对其未来发展方向进行了展望,以期为茄科作物改良提供理论参考。
Solanaceous crops,encompassing key food,vegetable,and economic crops such as potato(Solanum tuberosum),tomato(Solanum lycopersicum),pepper(Capsicum spp.),and tobacco(Nicotiana tabacum),are globally significant,and their yield and quality directly impact agricultural development and human health.However,solanaceous crops often face challenges from biotic and abiotic stresses during production,necessitating genetic improvement techniques like gene editing and transgenic technology to enhance their stress tolerance and yield.Agrobacterium rhizogenes-mediated transformation has become an important tool for molecular breeding and gene functional studies in solanaceous crops due to its broad host range,operational simplicity,lack of requirement for tissue culture,stable genetic inheritance,and suitability for functional analysis of root-related genes.This paper summarizes the classification and structural characteristics of the Agrobacterium rhizogenes Ri plasmid and its applications in solanaceous crops.It focuses on innovative practices of Agrobacterium rhizogenes in gene function validation and resistance breeding,and provides an outlook on its future development directions,aiming to offer theoretical references for the improvement of solanaceous crops.
作者
段宇
胡博文
DUAN Yu;HU Bowen(College of Horticulture,Hunan Agricultural University,Changsha 410128,China;Yuelushan Laboratory,Changsha 410128,China)
出处
《辣椒杂志》
2025年第3期11-19,共9页
Journal of China Capsicum
基金
湖南省重点研发计划(2023NK2006)。
关键词
发根农杆菌
茄科作物
转化体系
Agrobacterium rhizogenes
Solanaceae crops
transformation systems
