摘要
为探究外施脯氨酸(proline,Pro)对干旱胁迫条件下小麦苗期植株生长的影响,选用小麦品种百农207为试验材料,以加清水为对照(CK),分别设置20%PEG-6000溶液模拟干旱胁迫处理(PEG)、20 mmol·L^(-1)脯氨酸处理(PRO)及施用20%PEG-6000溶液+20 mmol·L^(-1)脯氨酸处理(PFG+PRO),共计4个处理,研究小麦苗期外施脯氨酸调控其抗旱性的生理机制。结果表明,干旱胁迫前,各处理叶片的SPAD值及丙二醛(malondialdehyde,MDA)、可溶性糖(soluble sugar,SS)、Pro含量均无显著差异;干旱胁迫后,与CK相比,PEG处理的SPAD值降低,MDA、SS、Pro含量升高,SOD、POD、P5CS基因的相对表达量增加,苗高、茎叶鲜重、茎叶干重、根干重显著降低,根冠比显著升高;与PEG处理相比,PEG+PRO处理的SPAD及SS、Pro含量显著提高,MDA含量显著降低。PEG+PRO处理在干旱胁迫1 d时,SOD、POD、CAT基因的相对表达量显著高于PEG处理;在干旱胁迫2 d时,LEA与P5CS基因相对表达量显著高于PEG处理。干旱胁迫后,PEG+PRO处理的株高、茎叶干重分别较PEG处理显著增加11.64%、12.96%。综上,小麦苗期外施20 mmol·L^(-1)脯氨酸处理可降低叶片MDA含量,提高叶绿素、SS、Pro含量以及抗逆相关基因SOD、POD、CAT、LEA和P5CS的相对表达量,从而提高小麦抗旱性,为小麦后期正常生长发育奠定基础。
In order to investigate the effects of proline(Pro)application on seedling growth of wheat under drought stress,wheat variety Bainong 207 was used as experimental material,and 4 treatments were set,including water(CK),20%PEG-6000 solution(PEG),20 mmol·L^(-1)proline(PRO)and 20%PEG-6000 solution+20 mmol·L^(-1)proline(PFG+PRO).The physiological mechanism of drought resistance in wheat treated with proline at seedling stage were analyzed.The results showed that,before drought stress,there were no significant differences in SPAD value and the contents of malondialdehyde(MDA),soluble sugar(SS)and Pro among 4 treatments.After drought stress,compared with CK,SPAD value of PEG treatment was decreased,the contents of MDA,SS,Pro were increased,and relative expressions of SOD,POD and P5CS genes increased,seedling height,stem and leaf fresh weight,stem and leaf dry weight and root dry weight in PEG treatment were significantly lower,and root to shoot ratio was significantly higher.Compared with PEG treatment,the SPAD value and the contents of SS,Pro were significantly increased,MDA content significantly decreased.After 1 d of drought stress,the relative expressions of SOD,POD and CAT genes in PEG+PRO treatment were significantly higher than those in PEG treatment.After 2 d of drought stress,the relative expressions of LEA and P5CS genes were significantly higher than those in PEG treatment.After drought stress,the plant height,stem and leaf dry weight of PEG+Pro treatment were significantly increased by 11.64%and 12.96%compared with PEG treatment.In conclusion,external application of Pro at seedling stage could reduce MDA content in leaves,increase SPAD value,the contents of SS,Pro and the relative expression levels of stress-related genes including SOD,POD,CAT,LEA and P5CS,which could improve drought resistance of wheat and lay a foundation for normal growth and development of wheat in the following stage.
作者
师焕婷
杨龙飞
唐华苹
王鹏飞
康国章
韩巧霞
SHI Huanting;YANG Longfei;TANG Huaping;WANG Pengfei;KANG Guozhang;HAN Qiaoxia(National Engineering Research Center for Wheat,College of Agronomy,Henan Agricultural University,Zhengzhou 450046,China;State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Sichuan Agricultural University,Chengdu 611130,China)
出处
《中国农业科技导报(中英文)》
北大核心
2025年第11期93-101,共9页
Journal of Agricultural Science and Technology
基金
西南作物基因资源发掘与利用国家重点实验室开放基金课题项目(SKL-KF202208)
河南省科技攻关项目(232102110208)
国家自然科学基金项目(31401302)。
关键词
冬小麦
外源脯氨酸
干旱胁迫
基因相对表达量
winter wheat
exogenous proline
drought stress
relative gene expression
