摘要
为探究外源脯氨酸如何调控干旱胁迫下番茄幼苗的生长和生理特性,采用脯氨酸对番茄幼苗进行叶面喷施处理,设置1个正常浇水的对照组和5个干旱并分别喷施不同浓度脯氨酸的处理组(0、15、30、45、60 mg·L^(-1)),研究番茄幼苗在干旱胁迫下施加脯氨酸后其生长状况、叶绿素含量、丙二醛含量和相对电导率的变化。结果发现,干旱处理后,随着外施脯氨酸浓度的增加,番茄幼苗的株高、茎粗、鲜质量、根冠比和叶绿素含量均明显提高,说明脯氨酸可以显著改善干旱胁迫下幼苗的生长状况,对促进幼苗生长有积极作用。通过测定丙二醛含量和相对电导率,发现随着外施脯氨酸浓度的增加,二者含量均呈下降趋势,说明脯氨酸能够有效降低干旱胁迫对番茄幼苗细胞膜的伤害,增强番茄幼苗的抗旱性。综上,适宜浓度外源脯氨酸可通过促进生长、增强光合作用和保护细胞膜来提高番茄幼苗抗旱性,本研究结果为干旱胁迫下番茄提质增效提供了技术参考。
In order to investigate how exogenous proline regulates the growth and physiological characteristics of tomato seedlings under drought,proline was used for foliar spraying of tomato seedlings,and one control group with normal watering and five treatment groups with drought and different concentrations of proline sprayed(0,15,30,45,60 mg·L^(-1))were set up to study the changes in growth conditions,chlorophyll content,malondialdehyde content and relative electrical conductivity of tomato seedlings after the application of proline under drought stress.The results showed that the plant height,stem thickness,fresh mass,root-crown ratio and chlorophyll content of tomato seedlings increased significantly with the increase of externally applied proline concentration after drought treatment,indicating that proline could significantly improve the growth condition of seedlings under drought stress and had a positive effect on promoting seedling growth.Through the determination of malondialdehyde content and relative conductivity,I found that with the increase of externally applied proline concentration,both contents showed a decreasing trend,indicating that proline can effectively reduce the drought stress on the damage of tomato seedling cell membranes and improve the drought resistance of tomato seedlings.In conclusion,the appropriate concentration of exogenous proline can enhance the drought resistance of tomato seedlings by promoting growth,enhancing photosynthesis and protecting cell membranes,and the results of this study provide a technical reference for improving the quality and efficiency of tomato under drought stress.
作者
闫姝婕
孔超
姜乐
张全艳
YAN Shujie;KONG Chao;JIANG Le;ZHANG Quanyan(School of Resources and Environment,Linyi University,Key Laboratory of Soil and Water Conservation and Environmental protection in Shandong province,Linyi,Shandong 276000,China)
出处
《天津农业科学》
2025年第6期51-58,共8页
Tianjin Agricultural Sciences
基金
国家自然科学基金青年项目(32202455)
山东省自然科学基金青年项目(ZR2022QC016)
大学生创新创业训练计划项(X202410452412)。
关键词
干旱胁迫
番茄
外源脯氨酸
drought stress
Solanum lycopersicum
exogenous proline
