摘要
目的探讨非编码RNA GATA结合蛋白6反义RNA1(lncRNA GATA6-AS1)和GATA结合蛋白6信使RNA(GATA6 mRNA)在卵巢癌中的表达及临床意义。方法前瞻性选取天津医科大学第二医院妇科2022年1月至2025年1月98例卵巢癌患者为研究组,另选取同期98例卵巢良性病变患者为对照组。采用实时荧光定量聚合酶链式反应法检测卵巢癌及卵巢良性病变患者血清及组织lncRNA GATA6-AS1和GATA6 mRNA表达水平;Pearson法分析卵巢癌患者血清lncRNA GATA6-AS1和GATA6 mRNA水平相关性;绘制受试者工作特征(ROC)曲线分析血清lncRNA GATA6-AS1和GATA6 mRNA水平对卵巢癌患者诊断价值。结果与对照组比较,研究组患者血清lncRNA GATA6-AS1和GATA6 mRNA水平降低(0.76±0.18比1.00±0.22、0.75±0.19比1.01±0.21),差异有统计学意义(P<0.05);Pearson分析研究组患者血清lncRNA GATA6-AS1和GATA6 mRNA水平呈正相关(r=0.61,P<0.05);与卵巢良性病变组织比较,卵巢癌组织lncRNA GATA6-AS1和GATA6 mRNA表达水平下降(0.65±0.15比1.01±0.20、0.59±0.12比1.01±0.24),差异有统计学意义(P<0.05);病理类型为浆液性卵巢癌组织lncRNA GATA6-AS1和GATA6 mRNA表达水平均低于黏液性卵巢癌组织(0.61±0.10比0.77±0.16、0.54±0.11比0.74±0.14),差异有统计学意义(P<0.05);卵巢癌组织lncRNA GATA6-AS1和GATA6 mRNA表达与肿瘤长径、国际妇产科联盟分期、分化程度、淋巴结转移、腹膜转移、腹水有关(P<0.05);ROC曲线表明血清lncRNA GATA6-AS1和GATA6 mRNA二者联合诊断卵巢癌曲线下面积为0.905,显著大于lncRNA GATA6-AS1(Z=2.45,P=0.014)和GATA6 mRNA(Z=2.16,P=0.031)单独诊断。结论卵巢癌患者血清及组织lncRNA GATA6-AS1和GATA6 mRNA表达水平下降,呈正相关,二者联合检测对卵巢癌诊断价值较高。
ObjectiveTo investigate the expression and clinical significance of non coding RNA GATA binding protein 6 antisense RNA1(lncRNA GATA6-AS1)and GATA binding protein 6 messenger RNA(GATA6 mRNA)in ovarian cancer.MethodsA prospective study was conducted on 98 patients with ovarian cancer treated at the Second Hospital of Tianjin Medical University,from January 2022 to January 2025,and 98 patients with benign ovarian lesions during the same period were selected as the control group.Real-time quantitative polymerase chain reaction(qPCR)was used to detect the serum and tissue expression levels of lncRNA GATA6-AS1 and GATA6 mRNA in patients with ovarian cancer and benign ovarian lesions.Pearson analysis was performed to assess the correlation between serum lncRNA GATA6-AS1 and GATA6 mRNA levels in patients with ovarian cancer.Receiver operating characteristic(ROC)curves were plotted to analyze the diagnostic value of serum lncRNA GATA6-AS1 and GATA6 mRNA levels in patients with ovarian cancer.ResultsCompared with the control group,the serum levels of lncRNA GATA6-AS1 and GATA6 mRNA in the study group were decreased:0.76±0.18 vs.1.00±0.22,0.75±0.19 vs.1.01±0.21,with statistically significant differences(P<0.05).Pearson analysis revealed a positive correlation between serum lncRNA GATA6-AS1 and GATA6 mRNA levels in the study group(r=0.61,P<0.05).Compared with benign ovarian lesion tissues,the expression levels of lncRNA GATA6-AS1 and GATA6 mRNA in ovarian cancer tissues were reduced:0.65±0.15 vs.1.01±0.20,0.59±0.12 vs.1.01±0.24,with statistically significant differences(P<0.05).In serous ovarian cancer tissues,the expression levels of lncRNA GATA6-AS1 and GATA6 mRNA were lower than those in mucinous ovarian cancer tissues:0.61±0.10 vs.0.77±0.16,0.54±0.11 vs.0.74±0.14,with statistically significant differences(P<0.05).The expression of lncRNA GATA6-AS1 and GATA6 mRNA in ovarian cancer tissues was associated with tumor size,International Federation of Gynecology and Obstetrics stage,differentiation degree,lymph node metastasis,peritoneal metastasis,and ascites(P<0.05).The ROC curve indicated that the combined diagnosis of serum lncRNA GATA6-AS1 and GATA6 mRNA for ovarian cancer had an area under the curve(AUC)of 0.905,which was significantly higher than that of lncRNA GATA6-AS1 alone(Z=2.45,P=0.014)and GATA6 mRNA alone(Z=2.16,P=0.031).ConclusionsThe lncRNA GATA6-AS1 and GATA6 mRNA in serum and tissue of ovarian cancer patients decrease,showing a positive correlation.The joint of the two has high diagnostic value for ovarian cancer.
作者
陈逢振
徐燕颖
董娜
田磊
Chen Fengzhen;Xu Yanying;Dong Na;Tian Lei(Department of Gynecology,the Second Hospital of Tianjin Medical University,Tianjin 300211,China;Department of Obstetrics and Gynecology,the Affiliated Hospital of Nankai University(Tianjin Fourth Hospital),Tianjin 300202,China)
出处
《中国医师进修杂志》
2025年第11期987-992,共6页
Chinese Journal of Postgraduates of Medicine
基金
天津市教委科研计划项目(2021KJ231)。
