摘要
为解析南瓜(Cucurbita spp.)嫁接提高冬瓜(Benincasa hispida(Thunb.)Cogn.)枯萎病抗性的分子机制,本研究以‘海砧1号’南瓜、‘铁柱2号’冬瓜作为材料,采用插接法嫁接或自接(自接指砧木与接穗均来自同一种材料)获得嫁接冬瓜(GW)、自接冬瓜(SW)和自接南瓜(SP)3种幼苗,将其种植在8.0×10^(5) CFU·g^(−1)尖孢镰刀菌接菌浓度土壤中,以0 CFU·g^(−1)接菌浓度土壤为对照,培养5天后采集根部和叶片样品进行转录组测序,分析差异表达基因。结果表明,在叶片与根部中分别筛选出284个与2个潜在的抗病基因。对其进行GO与KEGG分析发现,苯丙烷生物合成是嫁接冬瓜叶片与根部共有的代谢通路;利用DRAGO 3对潜在抗病基因进行结构域预测,预测出16个与植物抗病相关的基因,主要与模式触发免疫(pattern-triggered immunity,PTI)途径有关;对叶片与根部中所有转录组序列进行转录因子(Transcription factor,TF)分析,分别鉴定出2828个和5426个差异表达TFs,其中,共有的大量转录因子集中在MYB、b HLH、ERF与NAC等转录因子家族上。综上可推测,苯丙烷生物合成、MYB、b HLH、ERF与NAC等转录因子在防御尖孢镰刀菌侵染上发挥重要作用。
To investigate the molecular mechanisms by which pumpkin grafting enhances resistance to Fusarium wilt in wax gourd,pumpkin variety‘Haizhen 1’was used as the rootstock and wax gourd variety‘Tiezhu 2’as the scion in this experiment.Three types of seedling materials were produced through grafting:grafted wax gourd(GW)to pumpkin,self-grafted wax gourd(SW),and self-grafted pumpkin(SP).These graftings were cultivated in soil inoculated with Fusarium oxysporum at a concentration of 8.0×10^(5) CFU·g^(−1),with a control group using soil at 0 CFU·g^(−1).After five days of cultivation,root and leaf samples were collected for transcriptome sequencing to analyze differentially expressed genes(DEGs).The results showed that 284 and 2 potential disease-resistant genes were identified in the leaves and roots,respectively.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis revealed that phenylpropane biosynthesis was a common metabolic pathway in both the leaves and roots of grafted wax gourd.Using DRAGO 3 for domain prediction of the potential disease-resistant genes,16 disease resistance-related genes were predicted,mainly associated with the PTI(pattern-triggered immunity)pathway.Transcription factor(TF)analysis of all the transcriptome sequences in the leaves and roots showed that there were 2,828 and 5,426 differentially expressed TFs identified in the leaves and roots,respectively.A significant number of these TFs were concentrated in the MYB,bHLH,ERF,and NAC families.It is hence hypothesized that phenylpropane biosynthesis and transcription factors such as MYB,bHLH,ERF,and NAC play crucial roles in defending against Fusarium oxysporum infection.
作者
吴昊龙
符厚隆
符君玉
廖道龙
周斌
刘子凡
弭宝彬
WU Haolong;FU Houlong;FU Junyu;LIAO Daolong;ZHOU Bin;LIU Zifan;MI Baobin(School of Tropical Agriculture and Forestry,Hainan University,Danzhou,Hainan 571737,China;Institute of Vegetables,Hainan Academy of Agricultural Sciences,Haikou,Hainan 571100,China;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;Hunan Vegetable Research Institute,Changsha,Hunan 410125,China)
出处
《热带生物学报(中英文)》
2025年第6期829-841,共13页
Journal of Tropical Biology
基金
国家自然科学基金项目(32260752)
海南省重大科技计划项目(ZDKJ2021005)。
关键词
冬瓜
南瓜
嫁接
尖孢镰刀菌
转录组测序
wax gourd
pumpkin
grafting
Fusarium oxysporum
transcriptome sequencing
