摘要
为了快速高效地获得间型脉胞菌菌丝体蛋白,本研究通过单因素实验和响应面法对液态发酵间型脉胞菌的培养基组成成分和培养条件进行优化。结果表明:间型脉胞菌OK菌丝体蛋白的最佳培养基成分为蔗糖34 g/L,酵母提取物5 g/L、MgSO_(4)1.5 g/L、维生素B_(2)0.1 g/L;最佳发酵条件为发酵初始p H 6、发酵时间5 d、发酵温度28℃、发酵转速180 r/min。在此条件下的菌丝体蛋白质产量达3.64 g/L,比基础培养基的蛋白质产量(1.10 g/L)提高了230.91%。
To obtain the mycelial protein of Neurospora intermedia OK rapidly and efficiently,we optimized the corresponding medium composition and culture conditions for liquid fermentation through single-factor experiments and response surface methodology.As a result,the optimal medium components for mycelial protein were composed of sucrose(34 g/L),yeast extract(5 g/L),MgSO_(4)(1.5 g/L),and vitamin B_(2)(0.1 g/L).The optimal fermentation conditions were initial fermentation at pH 6,fermentation time for 5 days,fermentation temperature at 28℃,and fermentation rotation speed at 180 r/min.Through the improvement in this study,the mycelial protein yield could reach 3.64 g/L,which was 230.91%higher than the protein yield from the original basic medium.
作者
韩思雨
戴意强
王喆
何子茵
王道营
孙玲
印伯星
夏秀东
HAN Siyu;DAI Yiqiang;WANG Zhe;HE Ziyin;WANG Daoying;SUN Ling;YIN Boxing;XIA Xiudong(School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China;Institute of Agro-Product Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Shanghai Changing Biotechnology Co.,Ltd.,Shanghai 200120,China;Experimental Farm of Yangzhou University,Yangzhou 225009,China)
出处
《生物加工过程》
2025年第5期555-567,共13页
Chinese Journal of Bioprocess Engineering
基金
国家重点研发计划(2024YFF1106800)。
关键词
间型脉胞菌
液态发酵
培养基优化
培养条件优化
响应面优化
Neurospora intermedia OK
liquid fermentation
medium optimization
culture condition optimization
response surface optimization
