摘要
目的 探讨白头翁汤对溃疡性结肠炎(ulcerative colitis,UC)模型大鼠脑肠肽及炎症因子的调节作用。方法 将62只大鼠随机分为对照组(11只),模型组(11只),白头翁汤高、中、低剂量组(各10只)及美沙拉嗪组(10只)。采用高糖高脂饮酒法建立湿热证模型,除对照组外,其余组采用2,4,6-三硝基苯磺酸和乙醇诱导法建立UC模型。模型复制成功后,对照组大鼠给予生理盐水0.5mL灌肠,各给药组大鼠分别给予相应药物0.5mL灌肠,每日1次,连续7d。比较各组大鼠体质量、大便性状变化及疾病活动指数(disease activity index,DAI)评分;苏木精—伊红(hematoxylin eosin,HE)染色法观察大鼠结肠组织病理学变化;免疫组织化学法检测大鼠结肠组织中白细胞介素(interleukin,IL)-6、IL-10表达水平;Western blot法检测大鼠结肠组织中一氧化氮合酶(nitric oxide synthase,NOS)、乙酰胆碱转移酶(choline acetyl transferase,ChAT)、c-fos,血管活性肠肽(vasoactive intestinal peptide,VIP)蛋白表达水平;ELISA法检测大鼠肠道内容物中色氨酸羟化酶1(tryptophan hydroxylase 1,TPH1)表达水平。结果 白头翁汤显著增加UC大鼠体质量、结肠长度(P<0.05),升高IL-10、ChAT、VIP表达水平(P<0.05),降低DAI评分和IL-6、NOS、c-fos、TPH1表达水平(P<0.05);改善大鼠肠道UC样病理变化。结论 白头翁汤可能通过调节脑肠肽的表达及炎症因子的释放减轻UC大鼠肠道损伤。
Objective To investigate the regulatory effect of Baitouweng Decoction on brain-gut peptides and inflammatory factors in a rat model of ulcerative colitis(UC).Methods A total of 62 rats were randomly divided into control group with 11 rats,model group with 11 rats,mesalazine group with 10 rats,and high-,middle-,and low-dose Baitouweng Decoction groups(with 10 rats in each group).The method of high-sugar high-fat drinking was used to establish a model of damp-heat syndrome,and all rats except those in the control group were induced with 2,4,6-trinitrobenzenesulfonic acid-ethanol to establish a model of UC.After successful modeling,the rats in the control group were given enema with 0.5 mL of normal saline,and those in each administration group were given enema with the corresponding drug 0.5 mL,once a day for 7 consecutive days.Body weight,stool form,and disease activity index(DAI)score were compared between groups;HE staining was used to observe histopathological changes of the colon;immunohistochemistry was used to measure the expression levels of interleukin-6(IL-6)and interleukin-10(IL-10)in colon tissue,and a quantitative analysis was performed;Western blot was used to measure the protein expression levels of nitric oxide synthase(NOS),choline acetyltransferase(ChAT),c-fos,and vasoactive intestinal peptide(VIP)in colon tissue;ELISA was used to measure the expression level of tryptophan hydroxylase 1(TPH1)in intestinal contents.Results Baitouweng Decoction significantly increased the body weight and colon length of UC rat(P<0.05),upregulated the expression levels of IL-10,ChAT,and VIP(P<0.05),and reduced DAI score and the expression levels of IL-6,NOS,c-fos,and TPH1(P<0.05).It could also improve the UC-like pathomorphological changes in the intestinal tract of mice.Conclusion Baitouweng Decoction may alleviate intestinal injury in UC rats by regulating the expression of brain-gut peptides and the release of inflammatory factors.
作者
王婕
鲍炳州
石健
卢灿省
WANG Jie;BAO Bingzhou;SHI Jian;LU Cansheng(The First Clinical Medical College of Anhui University of Chinese Medicine,Anhui Hefei 230012,China;Department of Coloproctology,The First Affiliated Hospital of Anhui University of Chinese Medicine,Anhui Hefei 230031,China)
出处
《安徽中医药大学学报》
2025年第5期78-83,共6页
Journal of Anhui University of Chinese Medicine
基金
安徽中医药大学科研基金项目(2021ycylc18)
安徽省中医药传承创新科研项目(2024CCCX003)。
关键词
白头翁汤
溃疡性结肠炎
脑肠肽
血管活性肠肽
色氨酸羟化酶1
乙酰胆碱转移酶
Baitouweng Decoction
Ulcerative colitis
Brain-gut peptides
Vasoactive intestinal peptide
Tryptophan hydroxylase 1
Choline acetyltransferase
